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OpenSPR 1-Channel Operation Manual V4.1.01
3.4
Recommended Cleaning
3.4.1
Regular Cleaning
Regular cleaning of the OpenSPR is recommended to prevent cross-contamination of samples
within the fluidic lines. This is particularly important to perform before between switching
binding systems for analysis, or if sample contamination is suspected. Nicoya Lifesciences’
Maintenance Kit (MNT-1) provides all necessary solutions to perform the regular cleaning
procedures, outlined below.
Materials Required:
•
2 mL sodium dodecyl sulphate (SDS), 0.5%
•
2 mL Sodium Bicarbonate, 0.2 M
•
2 mL Sodium Hypochlorite, 0.5%
•
DI Water, filtered
•
Blank Chip
•
4x disposable syringes with blunt-end injection tips (one syringe designated for each
solution)
Procedure:
1.
Ensure there is at least 50 mL of DI Water present in Buffer Bottle 3, and at least 20 mL of
DI water is present in a separate accessible container. Empty the Waste Bottle before
commencing.
2.
Begin the prime sequence and load a blank into the OpenSPR sample holder. Continue to
prime the OpenSPR with DI Water from Buffer Bottle 3. Allow the flow rate to continue
at the default speed of 200 µL/min.
3.
During the prime procedure, rinse the injection port with at least 3 mL of DI water using
a designated disposable syringe.
4.
When the prime is complete, rinse the injection port with 2 mL of the 0.5% SDS solution.
While the solution is still within the OpenSPR, perform a bubble removal injection of this
solution. This will pass the cleaning solution through the flow cell and fluidic lines that are
exposed to user samples.
5.
Once the bubble removal sequence with the 0.5% SDS is complete, rinse the injection port
with 3 mL of DI water.
6.
Rinse the injection port with 2 mL of 0.2 M sodium bicarbonate. While the solution is still
within the OpenSPR, perform a bubble removal injection of this solution.
