NEW BRUNSWICK SCIENTIFIC BioFlo 4500, Manual To Operations

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BioFlo 4500 M1254-0050 User’s Guide
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2. Reduce the vessel pressure to 0 PSIG using the
BACKPRESSURE
REGULATOR
located in the upper left rear corner of the open piping
frame (manual pressure control), or set the setpoint to “O” in the
PRESSURE DISPLAY GAUGE
on the touchscreen
.
3. Assuming the port was previously sterilized, carefully remove the port
steam cap and aseptically attach a harvest vessel using silicone or
Tygon type tubing.
4. To open the drain, rotate the drain valve handle as far as it will allow
so it is facing the front of the vessel. When the valve is open, the word
OPEN
is visible on the end of the handle.
5. Draining of the liquid may be accomplished by gravity, a peristaltic
pump, or by using the vessel pressure to drive out the liquid from
inside the vessel.
6. After draining the vessel, close the drain valve by rotating the drain
valve 180
°
. In the closed position, the handle should be facing the
front panel console and the word
CLOSE
is visible on the end the
handle.
7. Break the harvest vessel connection by removing the connecting
tubing from the quick-connect fitting.
8. Put the stainless steel port steam cap back on the port and re-sterilize
the port.
6.4.2 Asceptic Harvest/Drain Method B
1. Prepare an appropriate harvest vessel fitted with tubing and a quick-
connect fitting. Sterilize if appropriate.
NOTE:
Refer to your local NBS parts distributor or sales office for quick-
connect options.
2. Autoclave harvest vessel and quick-connect fitting in the closed
position (121ºC, 30 minutes minimum, depending on vessel).
3. Remove steam port cap from the harvest/drain port and connect the
quick-connect fitting from the harvest vessel to the harvest/drain port.