NEW BRUNSWICK SCIENTIFIC BioFlo 4500, Manual To Operations

Page: 22 / 214

BioFlo 4500 M1254-0050 User’s Guide
22
1.2 Specifications
BioFlo 4500 Fermentor/Bioreactor
Vessel
Design Code The vessel conforms to the latest issue of ASME, Sect. VIII
Pressure Vessel Code. It is supplied with copies of the
Manufacturer's Data Report (U-3). Vessel includes the
ASME-Code Stamp.
Total Volume 20L 30L
Maximum Working Volume 15.0L 22.5L
Minimum Working Volume 5.0L 7.0L
Aspect Ratio (H:D) 1.98:1 2.03:1
Material All surfaces in contact with the culture liquid, including the
headplate and the jacket, are fabricated from type 316L
stainless steel
Finish Interior Mechanically polished to 15-20 Ra, Electropolish optional
Exterior Mechanically polished to 25-30 Ra, Electropolish optional
Geometry Cylindrical with hemispherical bottom dish
Dimensions 20L 9.48" ID X 18.81" H (24.08 cm ID X 47.48 cm H)
30L
10.79" ID X 22" H (27.40 cm ID X 55.88 cm H)
Viewing Window Rectangular, approx. 1¼" W X 8½" H (3.18 cm W X 21.6
cm H), above and below maximum working volume
Baffles Four removable baffles
Pressure
Rating Vessel 40 PSIG (2.7216 atm = 2.756 bar), tested at 1.5 times
Maximum Allowable Working Pressure (MAWP)
Jacket 50 PSIG (3.402 atm = 3.445 bar), tested at 1.5 times
Maximum Allowable Working Pressure (MAWP)
Design
Temp.
300
°
F (149
°
C)
Impellers Type (2) six-blade Rushton 316L stainless steel turbine impellers
mounted on the impeller shaft, removable and adjustable
along the length of the shaft, are standard. Marine blade,
pitched blade, medium lift basket and various other low
shear axial flow impellers are optional.
Diameter 20L: 3¾" (9.53 cm), 30L: 4-5/16" (10.95 cm)
OTR Capable of producing a minimum oxygen transfer rate
(OTR) of 350 mMoles of O
2
/L/hr at minimum vessel
pressure of 2 PSIG (0.1360 atm = 0.1378 bar)