NEW BRUNSWICK SCIENTIFIC BioFlo 4500, Manual To Operations

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BioFlo 4500 M1254-0050 User’s Guide
198
1. Turn off all power to the fermentor and close all service supply valves.
2. Inspect O-rings on the headplate and their mating surfaces for lack of
resilience, tears, cuts or other conditions that may interfere with sealing.
3. Check all fuses on the equipment to make sure the fuses and holders have not
corroded, and that the fuses are still good.
4. Check all controls and accessible items (switches, knobs, connections, pilot
lights and fuse holders) to make sure that they are properly tightened. Tighten
any component that is loose.
5. With steam
OFF
, open and examine steam traps. In order to clean them,
unscrew the top. Examine the interior. Remove any foreign matter from the
trap.
6. Open and clean all inline check valves that may see media from the vessel. If
your media is high in solids content, the inlet air check valve may need to be
cleaned more frequently.
7. Check all hand valves, on both the vessel and the piping skid, for tightness of
the valve packing (just beneath the handle), which should be tightened to
produce a slight drag on the valve handle when operated.
8. Open and inspect, clean or replace all pre-filters on services (i.e., steam, water
and air).
9. Remove vessel bottom harvest drain valve and inspect the gaskets and
packing for excessive wear.
10. Replace all O-rings on the
INOCULATION
,
ADDITION
,
HARVEST/DRAIN
and
SAMPLING PORTS
. Coat O-rings with light film of silicone grease.
11. Check airflow for any variation from the maximum, with vessel empty
and
with water in vessel.
12. Set back pressure regulator or pressure control loop for 20 PSIG (1.3 bar)
vessel pressure, then turn off the entire unit once this pressure is reached.
Note any vessel pressure decrease. If there is more than a 2 PSI (0.13 bar)
drop within one hour, check for leakage.
34.4 One-Year Intervals
Once every year, perform all the three-month checks and inspections plus the
following: