NEW BRUNSWICK SCIENTIFIC BioFlo 4500, Manual To Operations

Page: 180 / 214

BioFlo 4500 M1254-0050 User’s Guide
180
Figure 37: Addition Valve Detail
29.2 Addition & Inoculation
29.2.1 Method A
1. Prepare a pre-sterilized additive in an appropriate addition vessel fitted
with tubing and a fitting that allows easy attachment to the quick-
connect.
2. Reduce the vessel pressure to 0 PSIG, manually or at the touchscreen.
3. Assuming a previously sterilized port, carefully remove the
inoculation port steam cap and aseptically attach an inoculation
container using silicone or Tygon type tubing.
4. To open the inoculation valve, press the black polymer handles in
(toward the vessel) and turn clockwise. This brings the valve’s
entrance port forward into the vessel.
5. Add the liquid inoculum by gravity feed or via a peristaltic pump.
6. After inoculation, close the inoculation valve: press the black polymer
handles in and turn counterclockwise, then release.
7. Break the addition vessel connection by removing the connecting
tubing from the quick-connect fitting.
ADDITION
VALVE
STEAM INLET
VALVE
TO
CONTAMINATED
CONDENSATE
DRAIN
CAP
PURE STEAM