NEW BRUNSWICK SCIENTIFIC BioFlo 4500, Manual To Operations

Page: 133 / 214

New Brunswick Scientific Co., Inc. User’s Guide
133
There is a choice of two methods for calibrating DO zero point:
•
An electronic method
•
Sparging with nitrogen
17.1.1 Electronic Method to Calibrate DO Zero Point
•
Polarize the probe for a minimum of six hours.
•
At the touchscreen, press the
CAL. BUTTON
in the
GAUGE SCREEN
. The
CALIBRATION SELECTION WINDOW
will appear.
•
Select the DO control loop from the list by pressing on it.
•
Unplug the DO probe from the console.
NOTE:
Do not leave the DO disconnected from the fermentor for more than
one minute. Prolonged disconnection will result in depolarization of
the probe.
•
With the probe unplugged, set the “zero”. Ensure that the
SET ZERO
function is selected, then use the onscreeen keypad to enter the
appropriate value into the
ZERO STD
text box. Use the
Clr, +/-
BUTTON
to clear any unwanted information from the text box. Once
the desired number is entered, press the
SET ZERO
number.