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Instruction manual V3.1 02/2001
9.2 Potential sources of errors - elimination
9.
Trouble shooting
Problem
Cause
Corrective action
- Systematically try several
clearance angle adjustments,
until the optimum angle is found.
- Check if all levers are locked
and screws are tightened on
the specimen and knife holder
systems. Retighten the levers
and screws if necessary.
- Use a different part of the knife
edge or use a new knife.
- Use a different part of the knife
edge or use a new knife.
- Precool the specimen on a cold
plate.
- Cool the specimen in iced water
immediately before sectioning.
- Clearance angle adjustment;
systematically decrease the
clearance angle until the
optimum adjustment is obtained.
- Select lower sectioning speed.
- Select lower sectioning speed.
- Clearance angle adjustment;
systematically decrease the
clearance angle until the
optimum adjustment is obtained.
- Check if all levers are locked
and screws are tightened on
the specimen and knife holder
systems. Retighten the levers
and screws if necessary.
- Insufficient knife inclination;
consequently the clearance
angle is too small.
- Insufficient clamping of speci-
men and/or knife.
- Blunt knife.
- Blunt knife.
- Specimen too warm.
- Clearance angle too big.
- Sectioning speed too high.
- Sectioning speed too high.
- Clearance angle too big.
- Insufficient clamping of speci-
men and/or knife.
Thick/thin sections
The section thickness varies from
one section to another. In extreme
cases, sections are skipped
meaning that a section is not
obtained.
Compressed sections
The sections are extremely
compressed, wrinkled or jammed
together.
The knife "rings" on the cutting
stroke when sectioning hard
specimens. Sections exhibit
scratches and chatter marks.
Summary of Contents for RM2145
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