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Leica RM 2145 Rotary Microtome
8. Daily routine operation
8.10Sectioning
Always rotate the handwheel evenly. Adjust
the rotation speed to the hardness of the
specimen. Harder specimens should be sec-
tioned at a lower speed.
Select the sectioning mode
SECT/µm
.
Select the desired sectioning section thickness or
check the already selected thickness setting for
appropriateness.
For sectioning do not use the same area of the knife
edge that you have previously used for trimming.
For that purpose, adjust the knife edge laterally by
repositioning the knife holder on the knife holder
base with lateral adjustment option. If you work with
a knife holder system without lateral adjustment, re-
position the knife / blade itself within the knife
holder.
Select conventional sectioning or rocking mode
(
ROCK
).
To section, rotate the handwheel evenly in a clock-
wise direction or (in rocking mode) back and forth.
Prepare the sections.
8.9 Trimming
Select the trimming mode
TRIM/µm
.
Select the desired trimming section thickness or
check the already selected thickness setting for
appropriateness.
Pull the section waste tray (optional accessory) out
from under the microtome base plate.
Remove the knife guard from the knife edge.
Unlock the handwheel.
Use the TRIM STEP mode to approximate the speci-
men to the knife / disposable blade.
Trim the specimen by rotating the handwheel clock-
wise or select the rocking mode
ROCK
to trim the
specimen by moving the handwheel back and forth
over a short distance.
Stop trimming when reaching the desired section-
ing level of the specimen.
Summary of Contents for RM2145
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