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Instruction Manual

Leica RM 2145 V3.1 English 02/2001
Always keep this manual near the instrument!

Read carefully prior to operating the instrument!

Leica RM 2145

Rotary Microtome

Summary of Contents for RM2145

Page 1: ...Instruction Manual Leica RM 2145 V3 1 English 02 2001 Always keep this manual near the instrument Read carefully prior to operating the instrument Leica RM 2145 Rotary Microtome...

Page 2: ......

Page 3: ...ility whatsoever is accepted for any fi nancial loss or consequential damage caused by or related to compliance with statements or other infor mation in this manual Statements drawings illustrations a...

Page 4: ...30 8 5 Installing the knife holder 30 8 6 Inserting the knife or disposable blade 31 8 7 Adjusting the clearance angle 31 8 8 Orienting the specimen only with orienting specimen clamp fixture 32 8 9 T...

Page 5: ...pecimen and during breaks always lock the handwheel and cover the knife edge with the knife guard The handwheel can be locked in any position with the locking lever 1 on the right side of the microtom...

Page 6: ...fe holders are equipped with a permanent mov able knife guard 1 which allows to cover the entire cutting edge with the knife clamped in any position Knife holders N The knife guard of knife holder N F...

Page 7: ...the local ambient temperature Failure to allow for the required waiting time may cause severe damage to the in strument Do not operate the instrument in rooms with explosion hazard The safety devices...

Page 8: ...exchanging fuses only use replace ment fuses with the same specification as the original ones Refer to chapter 4 Technical data for the correct specifica tions Optional accessories Combined carrier w...

Page 9: ...ife edge 100 mm Weight w o accessories 38 kg Control panel Width 140 mm Depth 240 mm Height 95 mm Height in inclined position 125 mm Weight net 2 4 kg Microtome Type RM 2145 rotary microtome Sectionin...

Page 10: ...ere the handwheel only needs to be moved back and forth over a small distance in each direction The instrument also has a number of special functions such as the programmable horizontal specimen stop...

Page 11: ...tting device for mounting the combined carrier for magnifier illuminations or the microscope carrier Control panel Fig 11 1 Clamping lever for lateral adjustment of the knife holder base Section waste...

Page 12: ...board box 1 Take out the accessory box 2 and the instruction manual Remove the outer cardboard box 1 by lifting it up wards Fold down the lateral parts of the inner cardboard box 6 Take out the fixing...

Page 13: ...ory box 6 Lift the instrument 4 from the foam stabilizers 5 on the wooden pallet holding it at the front of the base plate and at the recessed grip at the rear Fig 12 2 see previous page Place the ins...

Page 14: ...rew located in the center hole of the handwheel with an Allen key size 5 3 Removetheliddingfoilfromtheautoadhesivecover 4 and place it onto the handwheel To disassemble proceed in reverse order 6 3 Si...

Page 15: ...e instru ment when operating it at an incorrect volt age setting The voltage selector is located in the voltage selector housing 3 above the mains switch 2 on the rear of the instrument The actual vol...

Page 16: ...ler one of the two screw driversintothesmallrecess 4 ofcover 5 andpush very carefully using the screwdriver as a lever to remove the housing Pull out the voltage selector housing 3 Remove the voltage...

Page 17: ...ains cables that are supplied together with the in strument Before connecting the mains cable check if the mains switch 3 at the rear of the instrument is in the OFF position O Select the mains cable...

Page 18: ...rear of the in strument Switching on the mains switch is followed by a beep The instrument initializes Next the software version is displayed in the four digit display for approximately 2 seconds Dep...

Page 19: ...de 7 Setting up the instrument LED indicating trimming thickness setting Counter Reset Adjusting knob STOP LED flashes during coarse feed forward operation is illuminated when front limit is reached L...

Page 20: ...sections Retr m Retraction value setting in m Disp off Display OFF The LED of the selected display mode is illuminated To change the display mode push DISP MODE un til the LED of the desired mode is i...

Page 21: ...stop i e it can be rotated endlessly in either direction Value adjusting itself is of course not endless and will only take place within the given set tingranges e g sectionthicknesssetting 0 25 60 m...

Page 22: ...ncrements from 10 to 20 m in 5 m increments from 20 to 50 m in 10 m increments from 50 to 100 m in 50 m increments from 100 to 600 m Turn the adjusting knob 1 to select a value within the setting rang...

Page 23: ...switched off the last horizon tal stop position that has been saved position remains archived If the POS is released before Sto is displayed the pre viously saved position is maintained Also if during...

Page 24: ...backward travel of the specimen press the corresponding button Once the button has been pressed the specimen will travel all the way to the rear limit stop position Tointerruptthemovement pressanyone...

Page 25: ...holdingdownoneofthedouble arrowcoarse feed buttons multiple consecutive TRIM STEPs are performed as long as the button is pressed Sectioning modes Sectioning can be done either by the conventional met...

Page 26: ...on value of 10 m is preselected To activate the retraction setting mode press DISP MODE the necessary number of times until the LED at Retr m is illuminated The 4 digit display reads the currently sel...

Page 27: ...activate retraction rotate the adjusting knob clockwise until the LED display reads the desired retraction value To deactivate retraction rotate the adjusting knob counterclockwise until the LED disp...

Page 28: ...longer You can continue to section STOP yellow is illuminated When the front limit is reached the specimen will not advanceanyfurther i e sectioningautomaticallystops To continue sectioning move the s...

Page 29: ...the left into the dovetail adapter 3 up to the limit stop To tighten the clamping mechanism rotate the clamping lever 1 clockwise 8 2 Assembling the specimen clamp directly to the fixture for specime...

Page 30: ...into the specimen clamp Please refer to chapter 11 Optional accesso ries on how to clamp the specimens into the individual specimen clamps 8 4 Installing the knife holder base To release turn the lev...

Page 31: ...the knife clamping mechanism 8 7 Adjusting the clearance angle The scale for the clearance angle adjustment 0 5 and 10 is located on the right side of the knife holder A corresponding reference line f...

Page 32: ...ication is illu minated Prior to the next section the speci men will advance by the retraction value plus the selected section thickness There is dan ger that specimen and knife will be damaged Rotate...

Page 33: ...ent re position the knife blade itself within the knife holder Select conventional sectioning or rocking mode ROCK To section rotate the handwheel evenly in a clock wise direction or in rocking mode b...

Page 34: ...imen at the upper end of the cutting stroke activate the mechanical handwheel locking Be extremely careful when handling micro tome knives and or disposable blades The cutting edges are extremely shar...

Page 35: ...on after switching on the instrument a loss of param eters has occurred Press any key All parameters are then set back to the default values You can continue with your normal working routine However d...

Page 36: ...oning speed Clearance angle adjustment systematically decrease the clearance angle until the optimum adjustment is obtained Check if all levers are locked and screws are tightened on the specimen and...

Page 37: ...rvice 1 Mains cable not properly connected 2 Mains fuses defective 3 Cable of control panel not properly connected 1 Spike in mains power supply caused loss of set parameters 1 Lithium battery exhaust...

Page 38: ...sistant to acetone or xylene Make sure that no liquids get into the interior of the instrument while cleaning Remove sectioning debris with a dry brush Pull out the section waste tray to empty Remove...

Page 39: ...echnical Service engineer Ask for a Service Contract after the warranty pe riod expires Clean the instrument on a daily basis Once a month lubricate the following parts with microtome oil no 405 which...

Page 40: ...t travelling upward or downward the adjustment is cor rect Readjustment will be required if the specimen head slightly rises or drops How to readjust The adjustment is made with the screw that is acce...

Page 41: ...correct specifications The mains fuses are located in the voltage selector housing 3 Remove the voltage selector housing 3 see chap ter 7 1 2 Changing the voltage selector setting Remove the fuses 4 I...

Page 42: ...mp This enables to clamp round specimens in the stan dard specimen clamp Turntheknurledknob 1 counterclockwisetomove the movable jaw 2 downward Insert the pin 3 of the vee insert 4 in the hole 5 of th...

Page 43: ...ertthefoilclampinthestandardspecimenclamp as shown Turn the knurled knob 1 clockwise to securely clamp the foil clamp in the standard specimen clamp Clamping of flat samples To clamp flat samples repl...

Page 44: ...ght or left Turn the knurled knob 1 clockwise to securely clamp the foil clamp in the standard specimen clamp 12 1 6Holder for round specimens The holder for round specimens is designed to accom modat...

Page 45: ...the specimen Introduce the special wrench 2 in the hole 3 and open the clamping jaws with the special wrench Place the specimen between the jaws Clamp the specimen by tightening with the special wren...

Page 46: ...1Knife holder base without lateral displacement The universal knife holder base without lateral dis placement function can be repositioned on the micro tome base plate only in north south direction 12...

Page 47: ...er together with the knife holder base forward or backward as appropriate To clamp turn the clamping lever 1 clockwise East West 12 2 2 only The lateral displacement feature of the knife holder base e...

Page 48: ...t to move the knife support bar via the height adjust ment screws to the lowest position to prevent dam age to the cutting edge while inserting the knife Unscrew the knife clamping screws 2 counter cl...

Page 49: ...bar Inserting the knife Turn the knurled wheels 6 on the right and left of the knife holder in opposite directions to the front to move the knife support bar via the height adjust ment screws to the l...

Page 50: ...o clamp the plate rotate the clamping lever 2 upwards Exchanging the back pressure plate Loosen the four screws 4 on the back of the knife holder with a no 4 Allen key Remove the back pressure plate 5...

Page 51: ...pressure plate 7 is adjusted with screw 9 which is located in a bore on the underside of the knife holder and can be reached from the rear side of the knife holder Insertadisposableblade withthecutti...

Page 52: ...e clamping lever 2 up Cleaning The clamping plate 7 can be removed for cleaning To remove the blade relocate the clamping lever 2 down Carefully remove the blade Pull out the clamping lever 2 Remove t...

Page 53: ...53 Leica RM 2145 Rotary Microtome 12 4 Section waste tray Insert the section waste tray from the front under neath the microtome base plate 12 Optional accessories Fig 53...

Page 54: ...oles on the underside of the carrier and tighten with a screwdriver To disassemble proceed in reverse order Mounting the carrier Turn the microtome off with the mains switch Remove the cover cap 4 Int...

Page 55: ...housing It also may be slewn to the side if required To do so slightly lift the carrier at front and move as desired The carrier may be adjusted in height by means of the knob 9 The inclination angle...

Page 56: ...ecess of the cover and push carefully to remove Pull out the voltage selector housing 13 Remove the fuses 14 Use fuses of the same specification only For correct fuse type see label on the carrier Mou...

Page 57: ...he hole of the carrier 2 Place the carrier 2 with the screw on the micro tome housing so that the screw is located in the hole of the fitting device inside the housing Tighten the screw 5 with an Alle...

Page 58: ...ed on the sample whilst the other may be di rected on the knife Sectioning with a microtome will inevitably cause vi brations which might be disturbing when using the microscope at great magnification...

Page 59: ...on the knife holder base with lateral displacement func tion clockwise Pull the lever s out of the knife holder base The universal knife holder without lateral dis placement function has a hole on the...

Page 60: ...Adjusting the clearance angle The scale for the clearance angle adjustment 0 5 and 10 is located on the right side of the knife holder A reference point is provided on the hand rest Torelease turnthe...

Page 61: ...h the knife holder and the hand rest forward or back ward as appropriate To clamp turn the clamping lever 1 clockwise East West only knife holder base with lateral displacement function The lateral di...

Page 62: ...the adjusting screws 3 and 4 com pletely Completely unscrew pressure piece 5 with a slot ted screw driver and remove it together with spring 5a and pin 5b Release eccentric bolt 6 rotating countercloc...

Page 63: ...ers or segment arc 0402 20369 Insert for Ralph knives for knife holder GD with clamping frame for 25 mm knives 0402 09996 Insert for Ralph knives for knife holder GD with clamping frame for 38 mm kniv...

Page 64: ...0 60 Hz 0502 29971 230 V 50 60 Hz 0502 29972 240 V 50 60 Hz 0502 29365 Microscope carrier including stereo zoom microscope eye pieces microscope adapter and fiber optical light guide w o cold light so...

Page 65: ...een subjected to a com prehensive quality control procedure based on our strictin housetestingstandardsinordertoensurethat the product complies with its technical specification The warranty conditions...

Page 66: ...66 Instruction manual V3 1 02 2001 Notes...

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