19
19.
Close the Lid and push and release the
Black Knob to actuate the UV flash.
Then lift the Lid, remove the Slide and
carefully peel away each strip of Tape
as described in
Steps 15
and
16
.
Figure 22
. Curing multiple sections.
Summary of the Instrumedics Frozen Sectioning Process
FIGURE 23.
To minimize ice-crystal
size, tissue is snap-frozen with a
chilled heat extractor.
Snap-freezing and embedding.
A blockholder is placed on the
Gentle Jane
snap-freezing device.
CryoGel
or other embedding media is dispensed onto the blockholder
and a tissue specimen is positioned on top. The chilled heat extractor is
placed in its holder and released. When the heat extractor contacts the
specimen, the tissue and embedding medium is snap-frozen into a flat
block. The plane of the flat block face minimizes trimming. When
specimen shape or orientation are essential, the tissue is snap-frozen
using the
Instrumedics CryoGel / Rubber Mold
method.
FIGURE 24.
A cold adhesive tape is
used to support the section as it is
being cut.
Cutting
After the block is trimmed, a cold adhesive tape is adhered to the block
face. The tape supports and captures the section as it is being cut,
eliminating the need for a brush or anti-roll device.
FIGURE 25.
The still frozen section,
Transfer to Slide
A cold adhesive-coated slide is placed on a temperature-controlled pad.
The adhesive tape is placed section-side-down on the adhesive-coated
slide, and is laminated to the adhesive layer using a cold roller.
