18
a)
fixed in the
Instrumedics Aqueous
Fixative
,
b)
freeze-dried or freeze-substituted in
the cryostat before “anhydrous”
fixation,
Note:
The section should be immersed
in the fixative
inside
the cryostat.
Fixation should then continue at room
temperature.
c)
melted or air-dried and fixed with
the fixative of choice for your
protocol.
Figure 20
. Technique for removing Tape from
Slide.
Steps for Multiple Sections:
17.
Multiple sections can be positioned on
one Slide by placing the Tapes
perpendicular to the Slide. For small
tissue specimens, the Tape can be cut
into narrower strips. Follow
Steps 5 - 9
.
18.
To cure the adhesive on the slide, lift
the Lid of the Mech and seat the Slide
on the Flash Tray. The Tape tabs will
overhang the Flash Tray.
Figure 21
. Processing multiple sections.
