12
•
The right LED should be red if it is labeled Power and green if it is labeled Oil Bath.
Use
Once a tissue specimen has been frozen and mounted in the microtome chuck, follow the
procedure outlined below.
Steps for a Single Section:
1.
If the Cryo-Vac-Away is installed,
position the collection nozzle at the
knife edge and step on the foot switch
to automatically remove debris during
the trimming sequence. If the Cryo-
Vac-Away is not installed, trim the
block to the desired depth to obtain a
full block face and remove the debris
from the knife. Set the micrometer for
the desired section thickness and cut
two or three sections to be sure a full
section will be cut.
Figure 5
. Block trimming and debris removal
.
2.
Take a pre-cooled, pouched, Adhesive
Coated Slide, and while holding it
inside
the cryostat chamber, remove
the glass slide from the pouch.
Figure 6
. Adhesive coated slide in pouch.
.
Nozzle
