14
6.
Hold the Tape up by the narrow tab
with the adhesive side towards the
block face. Position the Tape in front of
the trimmed block so that the bottom
edge of the exposed adhesive is aligned
with the bottom edge of the block.
Place the Tape on the block face
making sure that the bottom wider tab
of the Tape overhangs the knife.
NO
PORTION OF THE ADHESIVE
WINDOW SHOULD OVERHANG
THE BLOCK FACE.
TO AVOID MELTING THE
SURFACE OF THE TISSUE DO
NOT TOUCH THE PORTION OF
THE TAPE THAT COVERS THE
BLOCK FACE.
Figure 10
. Placing Tape on block face.
7.
Using the cold Hand Roller, laminate
the Tape to the face of the block by
moving the Roller up and down one to
two times and then left to right one to
two times. Some pressure is required
for good adhesion. When finished,
place the Hand Roller back to its
original position
inside
the cryostat.
Figure 11
. Laminating the Tape to the block face.