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The initial freezing was poor (slow), causing
large ice crystals to grow. The section was
taken deep in the tissue block where the
freezing is the poorest.
Snap-freeze tissue at -60
°
C or colder. Using
Instrumedics’
Power Jane
, or
Stand Alone
Gentle Jane
or
Dewar Jane
snap-freezers with
liquid nitrogen produces the best results.
Snap-freeze the thinnest practical specimen.
The sections uppermost in the block will have
the minimum ice crystal artifact.
Use Instrumedics’ Aqueous Fixative.
NOTE: Excellent results with freeze-
substitution followed by “anhydrous” fixation
will occur
only
if the ice crystal size is
minimal.
