22
3.
For very sensitive antigens:
DMF/Glut
Dimethylformamide
70 ml
25% Glutaraldehyde
1 ml
Add H
2
O to make up to
100 ml
Transfer the freeze-substituted slide directly from acetone to this fixative at +4
°
C for
30 to 60 seconds. This transfer must be made
within
the cryostat.
Following fixation, the slides are rinsed in water prior to staining.
REFER TO REAGENT MSDS FOR SAFE HANDLING
CryoJane Maintenance
ECU
•
NOTHING SHOULD BE PLACED OR STORED ON TOP OF THE ECU! Any spills
which enter the ECU may severely damage the electronic components.
•
To clean the ECU, wipe the surfaces with either a dry rag, or if necessary, an alcohol
wipe. ACETONE OR OTHER SOLVENTS WILL DAMAGE THE PLASTIC
SURFACES.
•
Fuses can be replaced by unscrewing the two small fuse holders in the rear of the
ECU. Fuses should be 1 AMP SLO-BLO and ceramic core wound, (available from
Instrumedics).
DO NOT OPEN THE ECU! Only personnel authorized by Instrumedics should
service the ECU. Unauthorized opening of the ECU will void all warranties.
Unauthorized persons will assume all risks and responsibilities.
Mech
•
All metal surfaces may be wiped with alcohol or chlorine solution.
•
The Flash Tray should periodically be cleaned with alcohol to remove any adhesive
build-up.
•
Care must be taken to avoid damaging the glass filter in the Flash Tray.
