ibidi Gas Incubation System – Silver Line
7
Maintenance
7.1
Disinfection and Cleaning
7.1.1
Wiping the Outer Surface
To clean the Gas Mixer and Humidifying Column from the outside, switch off the Gas Mixer and dis-
connect all cables and tubing. Leave the instrument to cool down for approximately 5 minutes. Use
only dry cloth or a cloth dampened with water (ultrapure) or common lab disinfection solutions that
are based on quaternary ammonium compounds (e.g., Barrycidal 36 or Pharmacidal). Fingerprints
can be removed using ethanol or isopropanol (70%) or lens/eyeglass cleaning wipes.
CAUTION
– 70% ethanol or isopropanol can be used to wipe the Humidifying Column
from the outside. Do not use any other organic solvents. Do not expose to ethanol or isopropanol
for an extended period of time. It can lead to damage of the Humidifying Column.
7.1.2
Cleaning the Column’s Inner Face
Microbial contamination in the Humidifying Column is usually not a problem since distilled water
and light-starved environments (microscopy rooms) do not support microbe growth. If, however,
contamination has occurred, disinfect the Humidifying Column by running the system with 3% hy-
drogen peroxide for 1 hour. Subsequently wash with water. The use of anti-microbial agents is not
recommended since they can be transported as aerosols and end up in the specimen.
7.2
Influence of Surrounding Temperature and Airflow
The surrounding, ambient temperature affects the temperature inside the microscopy chambers. De-
vices, such as computers and camera controllers, can significantly heat up small rooms. In this case,
we recommend equilibrating the room temperature to the typical experimental conditions at least
2–3 hours before starting the experiment.
Airflow can enhance the effect of temperature and humidity changes in the vicinity of the Incubation
Chamber. In cases where airflow (e.g., air conditioning) cannot be stopped, we recommend protect-
ing the microscope as much as possible from it.
Version 1.0 (June 1, 2022)
36
Instruction Manual
