ibidi Gas Incubation System – Silver Line
2
Intended Use
The ibidi Gas Incubation System – Silver Line provides defined gas control for live cell imaging
experiments. It is intended to be used with stage top incubators and can be applied on various
microscopes. The Gas Incubation System mixes the required gas ratios in the output and actively
humidifies this mixed gas output before the gas enters the incubation chamber.
Two versions of the ibidi Gas Incubation System – Silver Line are available: the ibidi Gas Incubation
System, CO
2
– Silver Line (#11921) for control of CO
2
concentration and the ibidi Gas Incubation Sys-
tem, CO
2
/O
2
– Silver Line (#11923) for control of both, CO
2
and O
2
. The O
2
version can additionally
downregulate the amount of O
2
in the gas mixture to create hypoxia. Both versions can control the
humidity and CO
2
concentration.
To provide a fully controlled stage top incubator the Gas Incubation System can be combined with
the ibidi Heating System – Silver Line.
3
Principle
Physiological Conditions in Live Cell Imaging
Cells react sensitively to changes in their environment. Factors such as temperature, humidity, and
CO
2
/O
2
levels significantly influence the outcome of cell culture assays. In order to achieve biologi-
cally relevant and reproducible results, it is crucial to maintain optimal conditions on the microscope
stage during live cell imaging experiments.
To enable high-quality live cell imaging under physiological conditions, the following parameters
need to be considered:
Environment:
• Temperature
• Carbon Dioxide (CO
2
) Levels and pH of the
Medium
• Humidity and Evaporation
• Oxygen (O
2
) Levels
Optical Aspects:
• Condensation
• Imaging Method and Photon Dose
Figure 1: Live cell imaging conditions.
The ibidi Gas Incubation System provides a controlled atmosphere of gas (CO
2
or CO
2
and O
2
) as well
as a defined humidity in the emitted gas stream that is flushed continuously through the incubation
chamber. Oxygen can be reduced during the experiment, enabling the user to perform hypoxia
experiments (CO
2
/O
2
version only).
Instruction Manual
15
Version 1.0 (June 1, 2022)
