ibidi Gas Incubation System – Silver Line
5.4.2
Incubation Parameters
Table 7: Recommended parameters for the operation of the ibidi Gas Incubation System – Silver Line
Parameter
Recommended Values
CO
2
(%)
5–10% depending on buffer
O
2
* (%)
20% (see Figure
Humidity (%)
90–95%
Flow rate
10 l/h
Column temperature (°C)
50°C
*for CO
2
/O
2
version only
CO
2
regulation
CO
2
is standardly set to 5% in cell culture experiments.
Humidity regulation
Humidity values at the lower end of the control range can only be achieved
using dried compressed air or with a compressed air pump in a very dry environment. The exact
value of the lower limit will vary depending on the amount of water vapor present in the input gases
used, the pressure, and the temperature of the measured environment.
Flow rate
At flow rates below 7 l/h, gas mixes may not reach the highest possible humidity. If this
is the case, augment the gas flow rate until the humidity is reached.
O
2
regulation/hypoxia (Gas Mixer for CO
2
and O
2
only)
To generate hypoxia conditions, set the
oxygen to the target value. The O
2
will be replaced by nitrogen (N
2
) until the set value is reached.
Please keep in mind that in a CO
2
/air mixture, the O
2
concentration will drop when the CO
2
concen-
tration is raised. The maximum oxygen concentration thus decreases according to the CO
2
value you
have chosen. Please see the graphic below for estimating the maximum O
2
concentration in your gas
mix for a set CO
2
concentration. For example, when setting the CO
2
concentration to the common
value of 5%, the maximum oxygen level is decreased to 20% O
2
(see Figure
Version 1.0 (June 1, 2022)
32
Instruction Manual
