ibidi Gas Incubation System – Silver Line
(Table continued)
Defective humidity sensor.
Blow exhaled air directly on the sensor. You
should see an increase of humidity. If the
humidity sensor is not giving readings, it is
likely defective. Contact
8.6
Humidifying Column
Error Description
Possible Cause
Possible Solution
Gas is not running through the Hu-
midifying Column (no gas bubbles).
If the humidity value is close to or
at the set point, gas will stop flow-
ing through the Humidifying Col-
umn.
Let flow reach the
set point. If flow is
stable at set point no
bubbles form.
Gas bubbles form in the Humidifying
Column even when all valves are shut
or the device is turned off.
Defective valve.
Contact
Please also see chapter
. Contact
for further troubleshooting support.
8.7
Focus not Stable
Focus drift is detrimental for most microscopy experiments, especially long duration time-lapse ex-
periments. Focus stability is mainly influenced by mechanical changes and temperature variations.
Follow these recommendations to keep your cells in focus:
• Switch on all components (e.g., heating, gas incubation, computer, or other equipment) at least
60 minutes before starting the experiment.
• After you put the sample onto the microscope, wait 30 minutes before starting a time-lapse
experiment to achieve temperature and immersion oil equilibration
• Keep the room temperature as stable as possible. Air conditioning should either be working
continuously or switched off.
• Do not change the temperature during the experiments. Avoid door/window openings, as this
could rapidly change the temperature.
• Eliminate all sources of mechanical vibrations. Use a damped table for your microscope.
6
If the experiment needs to be started immediately, either after placing the sample on the microscope or after closing the
lid, we recommend checking the focus for 20 minutes. In the first few minutes after starting the experiment, temperature
equilibration might influence the focus/z-position of the cells.
Version 1.0 (June 1, 2022)
42
Instruction Manual
