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3 - LIMITATIONS OF USE
• The
Gilson Pipetting Aid
may not be used for measuring substances, the vapours of
which damage the following plastics: PP, SI, ABS, EPDM, POM.
•
Gilson Pipetting Aid
may not be used where the risk of an explosion risk is present.
• Flammable liquids shall not be measured - in particular substances with flash- point below 0 °C
(ether, acetone).
•
Gilson Pipetting Aid
shall not be used for drawing acids with a concentration above 1 mol.
•
Gilson Pipetting Aid
shall not be used for drawing solutions with a temperature above 50 °C.
•
Gilson Pipetting Aid
may work in temperature range from +10 °C to +35 °C.
4 - PREPARING FOR USE
Before starting, check that the battery has not discharged. To check this, press the aspira-
tion button (fig. 1A) and watch to see if the low battery indicator lights up (fig. 1E). If the
indicator does not light up, the pipetting aid is ready for use.
If the low battery indicator lights up, the pipetting aid shall be left for charging (recommended
action), or the charger shall be connected and the work continued during charging.
The
Gilson Pipetting Aid
may be charged only with the original charger.
The mains voltage shall conform with the specification on the charger.
Charging shall be done in accordance with Chapter 8 of this instruction manual.
5 - ASPIRATING AND DISPENSING LIQUIDS
Attaching a pipette
Before attaching a pipette, check that the pipette is not damaged, has no dents or
sharp edges in the part to be gripped. Check that the part to be gripped is dry.
The pipette shall be gripped as close to the upper end as possible and carefully inserted
into the pipette holder until resistance is noticed (fig. 2A). Do not apply excessive force so
as not to damage thin pipettes and to avoid injury risk. A pipette that has been correctly
attached and sealed in the holder should not tilt to the side.
After attaching a pipette, hold the pipetting aid so as to keep the pipette in the vertical posi-
tion. The shape of the casing makes it possible to lay down the pipetting aid with a pipette
attached. After use, it is recommended not to leave the pipetting aid with a pipette attached
for a long period, for example overnight or over a weekend.
Do not lay the pipetting aid down, when there is liquid in the pipette.
Filling the pipette
Before aspirating, set the suction speed using the HIGH/LOW switch (fig. 1C):
• HIGH position - fast aspirating
• LOW position - slow aspirating
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The LOW position is recommended for pipettes with a volume up to 5 mL, and the HIGH
position for pipettes with a volume greater than 5 mL. Hold the pipetting aid so that the
pipette is in the vertical position, immerse the pipette end in the liquid to be aspirated (fig. 2B),
and press the aspiration button gently. The pipette filling speed depends on how far the
aspiration button is pressed. The further the button is pressed, the faster the liquid is aspi-
rated into the pipette.
Aspirating a slightly greater volume than required is recommended (due to the meniscus
above the required volume mark). Adjust the aspiration speed, particularly in the final filling
stage, so as not to overfill the pipette.
Setting the volume
After filling, dry the end of the pipette with absorbent paper (one that does not leave
impurities), so as to remove any liquid from the outside surface of the pipette. Then set the
required liquid volume precisely. Press the dispense button gently (fig. 2C), dispense the
excess liquid from the pipette until the meniscus of the liquid aligns exactly with the mark
on the pipette that indicates the volume to be dispensed.
Dispensing
Hold the recipient vessel in inclined position, place the pipette end in contact with the inside
wall, and press the dispense button gently (fig. 2C). The dispensing intensity may be
adjusted depending on how far the dispense button is pressed. The deeper the button is
pressed the faster the outflow of liquid from the pipette.
The
Gilson Pipetting Aid
has two dispense modes. The dispense mode is selected with
the GRAV/ BLOW switch (fig. 1D).
• GRAV position - the dispense is effected in gravity mode, which means that the liquid
flows out of the pipette by its own weight. The outflow speed is adjusted depending on
how far the dispense button is pressed.
• BLOW position - the dispense is effected as in the GRAV mode, but when the dispense
button is pressed to the end position, the pump is started and the dispense is complteted
by blowing the liquid from the pipette.
Warning!
In the GRAV mode, the pipette is not completely emptied because of the characteristics
of the pipettes used with the pipetting aid.
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6 - TROUBLESHOOTING
If the pipetting aid does not function correctly, you should determine the cause and remove
the fault, before continuing to use the pipetting aid.
ENGLISH
