CAVRO XP 3000, Operator'S Manual, Page: 93 / 134

Maintenance
5-3
10% Bleach Cleaning
To clean the pump with 10% bleach, follow these steps:
1 Make a solution of 10% bleach by adding one part of commercial bleach to nine
parts of water.
2
Prime the pump with the 10% bleach and allow the solution to remain in the pump
with the syringes fully lowered for 30 minutes.
3 After the 30-minute period, remove the reagent tubing from 10% bleach solution and
cycle all the fluid from the syringes and tubing into a waste container.
4
Prime the pump a minimum of 10 cycles with distilled or deionized water.
Periodic Maintenance
Tubing, syringe seals, and valves require periodic maintenance. If they become worn, you
are likely to notice these symptoms:
•
Poor precision and accuracy
•
Variable or moving air gap
•
Leakage
If any of these symptoms occurs and it is not obvious which component is causing the
problem, it is easiest and most economical to replace one component at a time in the
following order:
•
input and output tubing
•
plunger seal
•
valve
The frequency of replacement will depend on the duty cycle, fluids used, and instrument
maintenance.
Quality Control Assurance
Check the accuracy and precision of the XP 3000 on a regular basis.
Cavro recommends checking both accuracy and precision gravimetrically, using an
analytical balance with the capability to measure to 0.1 mg. Gravimetric measurements
should be corrected for the specific gravity of water at the ambient temperature.
The syringe can be checked by programming in the desired volume and determining the
weight of fluid dispensed.
To determine precision and accuracy, run a minimum of 20 replicates. The Mean,
Standard Deviation and Coefficient of Variation (see formula below) can then be
calculated. The calculations to determine accuracy must take into account the specific
gravity of water, which is dependent upon temperature. In addition, to prevent a false