29
AR (Antigen Retrieval) Option
To allow for selection of different antigen retrieval solutions (AR1, AR2, AR3 or for the substitution of antigen retrieval with Buffer,
an Enzyme or a Block.
�
AR1 is a high pH solution.
�
AR2 is a low pH solution.
�
AR3 is specific to ISH.
�
Some antibodies do not require antigen retrieval. In this instance, AR Buffer would be desired.
�
Some antibodies require enzyme digestion instead of antigen retrieval. In this instance, AR Enzyme would be desired.
�
If an antibody requires a block and does not require enzyme digestion or antigen retrieval, then AR Block would be desired.
�
Be sure to adjust the corresponding Temperature adjacent to the AR Option column to the desired temperature. See Antigen
Retrieval Temperature section below.
�
It is recommended to consult a Biocare representative if making changes to the AR setting.
Antigen Retrieval Temperature
�
Be sure to adjust the corresponding Temperature adjacent to the 'AR Option' column to the desired temperature.
�
The standard temperature for Antigen Retrieval is 101°C.
�
The low temperature for Antigen Retrieval is 95°C.
�
A temperature above 103°C is not possible.
