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Z-head
The Z-head is mounted on the Y-arm that moves along the horizontal X and Y axes inside the work platform of the instrument.
The following are components of the Z-head:
1. Extraction Probe
The Extraction Probe, also known as Z2 in the ONCORE System Software, is a Teflon-coated stainless steel probe used to extract
reagents from the slide chamber. The probe is washed internally and externally before each new reagent is aspirated. There is no need
for disposable pipette tips.
2. Dispense Probe
The Dispense Probe, also known as Z1 in the ONCORE System Software, is a Teflon-coated stainless steel probe used to dispense
reagents and buffer into the slide chamber. The probe is washed internally and externally before each new reagent is aspirated. There
is no need for disposable pipette tips.
3. 2-D Matrix Scanner
The 2-D Matrix Scanner is an electrical device used for reading the 2-D Matrix Code on the Slide Label. The 2-D Matrix Scanner consists
of an LED that integrates the unique 2-D Matrix Code into the ONCORE System Software.
4. RFID System
The RFID System is responsible for reading and writing to the Reagent Vial RFID Tag. The RFID System reads the reagent vial RFID tag
during reagent check to confirm the reagent name as well as the number of tests remaining in the reagent vial. The RFID System writes
information to a new Improv Reagent Vial RFID tag while in the RFID Editor.
Buffer and Waste Storage
The ONCORE Buffer and Waste Carboys have volume estimation markings on the applied label. Use these markings to estimate the
amount of liquid currently inside of the carboys. Prior to the start of each run, the software will display estimates of the required volume
of wash buffer as well as the amount of waste the run is expected to generate. If there is insufficient wash buffer in the ONCORE
Buffer Carboy connected to the buffer inlet or insufficient space in the ONCORE Waste Carboys, please correct this condition before
continuing with the staining process. It is recommended to fill the ONCORE Buffer Carboy and empty the ONCORE Waste Carboys prior
to starting a staining run.
Two separate 2-liter carboys are provided for non-hazardous and hazardous waste. The actual amount of waste volume will depend on
the number of reagents used. Waste lines are attached to the Hazardous Station and the Non-Hazardous Station respectively and are
fed through the base of the instrument to the waste carboys. Waste flows to both waste carboys via gravity.
Warning! There is no alarm for waste overflow. Waste carboys should be checked before each run to ensure enough capacity is
available for the volume of waste potentially generated. Be sure to use the secondary waste container provided.
