Bio-Tek ELx800, Operator'S Manual

Page: 154 / 214

126
|
Chapter 4: Performance Verification and IQ/OQ/PQ Procedures
1 Prepare the stock buffer solution using the ingredients in
Table 4 :
Table 4
PBS 10X Concentrate Solution
KH
2
PO
4
anhydrous 0.2 grams
NaCl 8.0 grams
Na
2
HPO
4
anhydrous 1.15 grams
KCl 0.2 grams
Tween
®
20 0.5 ml
Add DI water to bring to 100 ml
2 Mix 5 ml of the concentrated PBS solution with 45 ml of DI
water.
3 Add 10 mg of the
D
-NADH powder and mix thoroughly.
This is the 10x Concentrate PBS Solution .
Solution B: Sigma PBS
Required Materials:
•
Deionized water
•
Tween

20 (polyoxyethylene (20) sorbitan monolaurate)
• Sigma
®
P 3563 packets
•
D
-NADH Powder (
D
-Nicotinamide Adenine Dinucleotide,
Reduced Form) Sigma
®
bulk catalog number N 8129, or
preweighed 10-mg vials, Sigma
®
number 340-110
Store the
D
-NADH powder according to the guidelines on its
packaging.
1. Prepare a PBS solution using the Sigma powder.
2. In a beaker, mix 50 ml of the PBS solution (prepared from the
Sigma powder) with 10 mg of the
D
-NADH powder and mix
thoroughly.
This is the Sigma PBS Solution .
Bio-Tek Instruments, Inc.