Bio-Tek ELx800, Operator'S Manual

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Chapter 3: Operation
X/Y Axis Type
Note: LIN/LIN axes are recommended when using the Cubic Spline fit.
After the curve-fit type is selected, select the X/Y Axis Type.
X / Y A X I S T Y P E : L I N
L I N L I N / L O G L O G L O G / L I N
• Select the method by which the X- and Y-axes will be scaled.
•
This option is not available for the 2-P and 4-P curve-fit types. The X/Y
scaling for these curves is always LIN/LIN.
Extrapolation of Unknowns
This screen allows you to choose whether to extrapolate the curve to evaluate
samples outside of the absorbance range defined by the standards.
E X T R A P O L A T E U N K N O W N S ? Y E S
Y E S N O
•
Select
YES
to enable extrapolation; otherwise, select
NO
.
• On the printed reports, extrapolated concentrations (RSLT values) are
surrounded by
< >
(e.g.,
<44.425>
).
If extrapolation is chosen for the Point-to-Point curve
fit, unknown concentrations will be extrapolated
linearly from the nearest segment of the curve. If the
plot includes both increasing and decreasing segments,
the curve printout will be labeled “Ambiguous.” The
resulting values, which actually are extrapolated, may
not be indicated as such. All calculated results for an
“Ambiguous” curve should be considered unreliable.
Bio-Tek Instruments, Inc.