Bio-Tek ELx800, Operator'S Manual, Page: 103 / 214

Define (Method, Map, Formula and Curve)
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75
Transformation Formulas
Transformation formulas can be used to transform raw or blanked absorbance
data in preparation for further data reduction, including curve-fit analysis.
See Formula Type on page 70 for instructions on selecting an assay and
accessing the Transformation Formula definition screen.
• If a blanking method is selected in the assay, transformation formulas
are applied to the blanked absorbance values; otherwise, they are
applied to the raw data. Turn to page 69 to review the results
calculation structure.
• One transformation formula may be defined per assay.
• A transformation formula can be simple (ex.
(OD/2)*100
to multiply
all wells on the plate by 100), or more complex with the inclusion of a
predefined Transformation Variable (see
TVAR , below).
Simple Transformation Formulas
“Simple” transformation formulas are typically applied to all wells on the
plate. For example:
To divide the OD in each well on the plate by 2 and then multiply
by 100, the formula is:
(OD/2)*100
Transformation Variable (TVAR)
For more complex transformations, a Transformation Variable (TVAR) can
be defined for use within a transformation formula. This variable defines
the scope of the transformation: whether to apply the transformation to all
of the wells on the plate (OD), or just to the sample wells (SMP).
S C O P E V A R I A B L E : O D
S M P O D
ELx800 Operator’s Manual