Bio-Tek ELx800, Operator'S Manual

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Chapter 4: Performance Verification and IQ/OQ/PQ Procedures
8. The standard deviation for each set of readings should be less than
the allowed deviation.
For example: Absorbance readings of 1.950, 1.948, 1.955, 1.952, and
1.950 will result in a mean of 1.951, and a standard deviation of
0.0026. The mean (1.951) multiplied by 0.5% (1.951 * 0.005) = 0.0098,
which, when added to the 0.005 (0.0098 + 0.005) = 0.0148 OD, which
is the allowable deviation. Since the standard deviation is less than
this value, the reader meets the test criteria.
Repeatability Specification:
For comparison in this test, the following repeatability specification
is applied, using the Normal Read Mode and a 96-well microplate.
± 0.5% ± 0.005 OD from 0.000 to 2.000 OD @ 405 nm
Bio-Tek Instruments, Inc.