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Aquasafe WSL50 Pro, Instruction Manual

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Aquasafe WSL50 Pro Instruction Manual Download Page 17

 

 

 

3.0 Operating instructions 

3.1 Incubator controls 

 

 

 

 

 

 

 

Petri-Lok® Cassette.  

The Petri-Lok® cassette consists of a rack and a spring 
loaded top to hold the petri-dishes in place. To 
remove the cassette twist the cassette to the left and 
lift out.  The top of the cassette is removed by pushing 
down on the top whilst holding the rack, twisting to 
the left and then removing the top. To re-fit align the 
arrow with either of the two slots in the top of the 
rack, push down and twist lock into place.  

The incubator has a power connection socket on the 
rear of the unit and three switches on the top which 
control all functions of the incubator.  

The incubator can be run from a power supply in the 
range 12 – 24 Volts.  

All information is displayed to the user via the LCD 
display on the top of the unit.  

Summary of Contents for WSL50 Pro

Page 1: ...AquaSafe WSL50 Pro Water Safety Laboratory Instruction Manual ...

Page 2: ...s Section 1 Introduction Section 2 Kit Contents Section 3 Double Incubator Instructions Section 4 Membrane Filtration Instructions Section 5 HydroTest HT1000 Instructions Section 6 Multi parameter Instructions ...

Page 3: ...Integrated dual chamber digital incubator complete with all accessories and consumables for carrying out 300 tests for Faecal and Total Coliforms Photometer kit complete with all accessories and consumables for 300 tests of Ammonia Free Total Chlorine Nitrate and Nitrite Multi parameter electrochemical meter kit complete with electrodes and calibration solutions for testing pH conductivity and TDS...

Page 4: ...Section 2 Kit Contents c c c c b a e f d ...

Page 5: ...1 Dilution Tube c 4 Sample Collection Bottles with Dechlorination Tablets d 1 5ml Sterile Syringe e 1 30ml Dropper Bottle for Methanol f 1 Membrane Filtration Unit including water beaker sample beaker measuring funnel sintered glass disk 1 silicone gasket g 1 Sterile Membrane Filters 100 Pack h 1 Forceps i 1 Vacuum Tube j 1 Eyeglass k 1 Vacuum Pump l 2 Sterilised Water for MLSB Preparation m 1 Pad...

Page 6: ...Check 1 EC Probe for HydroCheck 1 Thermal Probe for HydroCheck 1 pH 4 01 Buffer Solution 1 pH 7 00 Buffer Solution 1 EC 1413µS Calibration Solution Separate Box Qty Description 3 Ammonia Reagents 100 Pack 3 Chlorine Free Total Reagents 100 Pack 3 Nitrite Reagents 100 Pack 3 Nitrate Reagents 100 Pack 3 Arsenic Economy Test Kit 100 Tests 4 Sterilised Water for Membrane Lauryl Sulfate Broth Preparati...

Page 7: ...Section 3 ...

Page 8: ...AquaSafe Micro biological Incubator Instruction Manual ...

Page 9: ...ber 20 3 3 2 Menu sequence Right Chamber 22 3 3 3 Start screen Figure 1 1 23 3 3 4 37 C Program Fig 1 2 24 3 3 5 44 C Program Fig1 3 25 3 3 6 User defined Program Fig 1 4 26 3 3 7 Resetting the timer Fig 1 5 27 3 3 8 Incubation cycle time setting Fig 1 6 27 3 3 9 User defined temperature setting Fig1 7 28 3 3 10 Calibration Fig 1 8 29 4 0 Care and Maintenance 30 4 1 General 30 4 2 Cleaning 30 4 3 ...

Page 10: ...6 0 Guarantee and Assistance 32 ...

Page 11: ...m x 3 5mm aluminium petri dishes which are suitable for 47mm membrane filter pads but the incubator can also be used with 55mm pre prepared plastic petri dishes The incubator has two independently controlled chambers each supplied with 25 Petri dishes as standard but have the capacity for more due to the Petri Lok system The incubator has the option to run 37 C 44 C and user defined temperature pr...

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Page 13: ...such that it cannot become depressed during transit covering the recessed plate with rigid card should achieve this 2 1 Docking Station The Incubator is mounted on a docking station built into the carry case which incorporates the rechargeable battery pack and allows the incubator to be run from the batteries during transportation It is also possible to use the incubator on a bench independent of ...

Page 14: ...rect connection of the wires will invalidate the warranty It is also advised that batteries are only removed if absolutely necessary 2 3 Battery Charging It is recommended to charge the battery fully prior to use To do this remove the power supply unit from the kit and connect it to the charging connection on the rear of the carry case screwing firmly into position Plug the other end into a 90 240...

Page 15: ...incubator is used to prevent discharge of the vehicle battery Alternatively an auxiliary battery may be used Cable available separately Note The vehicle cigarette lighter charging lead is a specialist product and as such only a genuine Trace2o replacement should be used Replacements are available from any Trace2o approved representative No attempt should be made to charge or power the equipment ot...

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Page 17: ... the top whilst holding the rack twisting to the left and then removing the top To re fit align the arrow with either of the two slots in the top of the rack push down and twist lock into place The incubator has a power connection socket on the rear of the unit and three switches on the top which control all functions of the incubator The incubator can be run from a power supply in the range 12 24...

Page 18: ...the incubation cycle from where it was before the power was removed Mode 3 is primarily intended for unattended power failure conditions If Fig 1 0 is displayed and the incubator was in mode 2 then the previous incubation cycle can be restarted from where it was left by pressing restart This will be discussed later The screen will be displayed for 60 seconds before defaulting to the restart condit...

Page 19: ...used to step through the sequence instead of the left As such the functions of the right chamber will not be described in detail It should be noted that each chamber can be run with a different setting as they are independent of each other however when the incubator is in run mode changes cannot be made to the settings of either chamber To make changes to the setting of one or both chambers the us...

Page 20: ...or the left chamber The bottom right of the display will show the current state of the right chamber OFF is shown for illustration purposes only 20 9 C 20 9 C OFF MEM OFF Select Option Restart NewRun 20 9 C 20 9 C 37 MEM OFF 20 9 C 20 9 C 44 MEM OFF 20 9 C 20 9 C U MEM OFF 20 9 C 20 9 C New SEL OFF 20 9 C 20 9 C Cal ADJ OFF 20 9 C 20 9 C U ADJ OFF 20 9 C 20 9 C Tim ADJ OFF Fig 1 0 Fig 1 1 Fig 1 2 ...

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Page 22: ...iddle switch will select this action for the right chamber The bottom left of the display will show the current state of the left chamber OFF is shown for illustration purposes only 20 9 C 20 9 C OFF MEM OFF Select Option Restart NewRun 20 9 C 20 9 C OFF MEM 37 20 9 C 20 9 C OFF MEM 44 20 9 C 20 9 C OFF MEM U 20 9 C 20 9 C OFF SEL NEW 20 9 C 20 9 C OFF ADJ Cal 20 9 C 20 9 C OFF ADJ U 20 9 C 20 9 C...

Page 23: ...3 3 3 Start screen Figure 1 1 This screen shows the current status of the incubator In the state shown the incubator is in idle mode and not running in either chamber 20 9 C 20 9 C OFF MEM OFF ...

Page 24: ...return to the menu function screen During the run mode a short press of the middle switch will indicate the battery voltage For best accuracy always check the battery voltage when the heater is off when the asterisk is not displayed Once the chamber has reached the pre set temperature the timer will start to countdown If for any reason the temperature should drop such as power failure the timer wi...

Page 25: ...5 seconds will start the incubation cycle and the screen will change to the following The asterisk preceding the temperature will switch on and off indicating the status of the heater To stop or pause the incubation cycle press and hold the middle switch again for 5 seconds The display will show the following Pressing and holding the middle switch will restart the cycle or pressing the left switch...

Page 26: ...middle switch for 5 seconds will start the incubation cycle and the screen will change to the following The asterisk preceding the temperature will switch on and off indicating the status of the heater To stop or pause the incubation cycle press and hold the middle switch again for 5 seconds The screen will display the following Pressing and holding the middle switch will restart the cycle or pres...

Page 27: ...d then turn back on which will exit this mode and return to the menus without resetting the timer 3 3 8 Incubation cycle time setting Fig 1 6 In this mode the default cycle time can be changed from 24 hours to anywhere between 0 and 24 hours Before adjusting the cycle time reset the timer as described in section 3 3 7 To adjust the time press the middle switch The screen will change to Pressing th...

Page 28: ...To adjust the temperature press the middle switch The screen will change to Use the left switch to decrease the temperature and the right switch to increase the temperature When the desired temperature is set press and hold the middle switch to save the settings The display will return to the one shown in Fig 1 1 20 9 C 20 9 C U ADJ OFF C User Temp Save ...

Page 29: ...our Compare the displayed temperature on the incubator screen to that shown on the thermometer If there is a difference 0 2 C the calibration should be adjusted as follows Exit the run mode and step through the screens until the calibration screen is displayed Press the middle switch to enter calibration mode The following screen will be displayed Adjust the temperature up or down using the left a...

Page 30: ...wet conditions and must not be submersed in water The incubator chamber is sealed at the bottom so spills will not affect the unit but should be cleaned as soon as possible The Aluminium chamber can be cleaned with Alcohol soap or solvent based cleaners Do not use abrasive or chlorine based cleaners The Petri Lok rack can be steam sterilised if required but it is not recommended to steam sterilise...

Page 31: ...he rechargeable lead acid batteries may be necessary If so these must be replaced with batteries of the same type as specified in section 5 1 and replaced as a pair Should any fault occur please contact the Trace2o Technical Team who will be more than happy to advise you 5 0 Technical Specification 5 1 Docking station Power supply Input Max 12 28V DC 48W via supplied Power supply unit or vehicle c...

Page 32: ...nd Assistance Trace2o hope that the AquaSafe incubator will give many years of trouble free operation but in the event of a technical problem occurring the AquaSafe incubator is covered by Trace2o Ltd s standard Guarantee terms and conditions available via email or via download from www trace2o com In the event that any technical assistance is required the Trace2o Customer Service department will ...

Page 33: ...Section 4 ...

Page 34: ...AquaSafe Membrane Filtration Instruction Manual ...

Page 35: ... 38 SECTION 3 PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD 39 SECTION 4 SAMPLING 40 SECTION 5 USE OF BACTERIOLOGICAL MEDIA 42 SECTION 6 ASEPTIC PROCEDURES 43 SECTION 7 PROCESSING SAMPLES FOR COLIFORM ANALYSIS 45 SECTION 8 COUNTING COLIFORMS AND RECORDING THE RESULT 48 SECTION 9 SELECTING THE OPTIMUM VOLUMES FOR MEMBRANE FILTRATION 2 ...

Page 36: ...ASSEMBLY OF FILTRATION APPARATUS Hand Vacuum Pistol Pump Filtrate Flask Waste Beaker Sampling Cup and cable Graduated Aluminium Funnel Membrane Support Holder Sealing Gaskets Glass Sintered Disc Membrane Support ...

Page 37: ...r and press into place Place the sintered disc smooth side facing upwards into the centre of the gasket Push the other two gaskets into place around the sintered support disc The graduated funnel screws clockwise into position Do not over tighten the funnel ...

Page 38: ...r cleansing agent pour the prepared medium carefully into the bottles Replace bottle lids but leave them slightly loose do not fully tighten Sterilise in an autoclave at 121 C for 10 minutes or place bottles in a pressure cooker and maintain in steam at pressure for 15 minutes Remove the bottles allow them to cool to room temperature fully tighten the tops and then store in a cool dark place When ...

Page 39: ...ange to green to indicate moisture penetration Tear open the sachet and remove the silica gel with the forceps If the silica gel is green discard the sachet without using Open a bottle of Sterilised water for broth preparation and carefully pour the entire contents of the sachet into the bottle Replace the bottle lid tightly and vigorously shake the mixture to ensure that the powder is fully disso...

Page 40: ...mple collection bottles contain dechlorination tablets N B If the sample is analysed immediately on site then it is not necessary to dechlorinate the water Dechlorination Kit The dechlorination tablets are pre placed inside each sample collection bottle Simply pour the sample from the sampling cup into the sample collection bottle cap and shake thoroughly to ensure thorough dissolving of the table...

Page 41: ...until the water cools to its original temperature Fill the prepared bacteriological sampling bottle leave a slight air gap at the top and seal the lid tightly Dip Sample Sterilise the sampling cup by igniting 1ml of methanol alcohol in the cup Allow to cool Rinse the alcohol from the cup with water from the sample source Immerse the cup into the water source to obtain the sample Pour into the prep...

Page 42: ... in a water bath for 15 minutes Clean empty media bottles thoroughly before re use Any residues should be washed out with hot water cleaned with a little detergent a small brush can be used if required rinsed several times in clean water dried and stored in a clean environment with the lids lightly attached The MLSB solution may be applied to the pads up to 6 hours before sampling if the pads are ...

Page 43: ...r ignite the methanol TAKE CARE AROUND THE NAKED FLAME Allow the methanol to burn for a few seconds Whilst the methanol is still burning invert the filtration unit and carefully place inside the sample cup Wait for at least 20 minutes to ensure that the sample cup and filtration unit are sterile Methanol burns anaerobically to form formaldehyde gas which reaches all areas of the filtration apparat...

Page 44: ... is necessary to dispense pads in the field great care must be taken not to contaminate either the pad dispenser or the cartridge As soon as a cartridge is finished a new one should be attached to the dispenser Do not leave the dispenser unattached for any length of time If no pad dispenser is available use sterilised forceps Before handling a membrane filter with the forceps the forceps should be...

Page 45: ...te the pad with prepared broth Loosen the graduated filter funnel and remove from the base support Sterilise the forceps and allow to cool Using these forceps place a sterile membrane onto the glass membrane support grid side up If the membrane tears or becomes contaminated discard it and use a new one Lock the membrane in place by screwing the filter funnel down into position ...

Page 46: ...r the water sample into the filter funnel up to the 100 ml graduation Connect the hand vacuum pump to the filtration unit base and pump in a controlled fashion to suck the water sample through the membrane ...

Page 47: ...ple number place date time etc Place the petri dish into the PetriLok cartridge Repeat the process for all the samples and then place the filled rack into the incubator It is important to note that when the last sample has been processed a resuscitation period of at least one hour but not more than four hours must be observed before incubating This allows any physiologically stressed coliforms to ...

Page 48: ...thin a few minutes of removing from the incubator as the colours are liable to change on cooling and standing Ignore those colonies that are not yellow e g pink transparent colonies Once the number of yellow colonies has been determined and assuming that 100 mL of sample was filtered this number of colonies equals the number of coliforms per 100 mL Where samples were incubated at 35 C the count is...

Page 49: ...s indicated If the count exceeds 10 faecal coliforms per 100 mL action is urgently required Raw Waters For source waters and partially treated waters including those which are ground water derived it can be useful to adjust the sample volume in order to obtain faecal coliform counts in the optimum range 10 200 It may also be useful to process more than one quantity on the first occasion a particul...

Page 50: ...Section 5 HydroTest Photometer HT1000 ...

Page 51: ...er Countdown 10 2 2 4 Display Backlight 11 2 3 Operation Mode 11 2 3 1 Automatic Switch Off 11 2 3 2 Selecting a Method 12 2 3 3 Method Information F1 12 2 3 4 Chemical Species Information 13 2 3 5 Differentiation 13 2 3 6 Performing Zero 14 2 3 7 Performing Tests 15 2 3 8 Changing Chemical Species 15 2 3 9 Storing Results 16 2 3 10 Printing Results T2o PC Link Optional 17 2 3 11 Performing Additi...

Page 52: ... 4 5 Lab Function 44 2 4 6 User Options 46 2 4 7 Special Functions 59 2 4 8 Instrument Basic Settings 2 62 2 4 9 Instrument Special Functions Service 64 2 5 Data Transfer 64 2 5 1 Data Printing 64 2 5 2 Data Transfer to a Personal Computer 64 3 0 Enclosure 65 3 1 Unpacking 65 3 2 Technical Data 65 3 3 Abbreviations 67 3 4 Troubleshooting 69 3 4 1 Operating Messages in the Display Error Display 69 ...

Page 53: ... Standard Methods for water analysis This instruction manual outlines the functions and operations of the photometric instrument only Instructions specific to each test are included with the reagent packs as purchased from Trace2o Ltd The instrument has been designed and optimised to work solely with Trace2o reagents A complete list of available parameters is included in Appendix 1 0 The instrumen...

Page 54: ...battery changeover time exceeds 2 minutes all the data and settings stored on the device are lost It is recommended to have the replacement batteries easily accessible to ensure quick changeover 2 1 3 Replacement of Batteries Ensure to read Chapter 2 1 2 before replacing batteries See Chapter 2 1 4 for an annotated diagram of the instrument 1 Switch the instrument off 2 If necessary remove vial fr...

Page 55: ...B Battery Compartment Cover C Notch D Batteries 4 x AA LR6 E Seal Ring F Instrument Back CAUTION To ensure that the instrument is waterproof Seal ring E must be in position Battery compartment cover B must be fixed with the four screws ...

Page 56: ...1 Function 1 key description in the text if key available Function 2 key description in the text if key available Function 3 key description in text if key available Displaying date and time user countdown Storing of displayed test result Menu of photometer settings and further functions Moving cursor up or down Performing Test Returning to selection of methods or previous menu Confirming ...

Page 57: ... 3 User Countdown With this function the operator is able to define their own countdown Press clock key The display shows time and date Press clock key again The display shows Either press key to accept the last user countdown Or press any number key to start entering a new value The entry comprises two digits each for minutes and seconds 19 30 22 2012 06 15 19 30 22 2012 06 15 Countdown mm ss 99 ...

Page 58: ...witched off automatically during the measurement 2 3 Operation Mode Switch the photometer on by pressing the ON OFF key The photometer performs an electronic self test 2 3 1 Automatic Switch Off The instrument switches off automatically after 20 minutes This is indicated 30 seconds before by a beeper Press any key to avoid the instrument switching off As long as the instrument is working for examp...

Page 59: ...irectly e g Shift 8 0 to select Bromine 2 3 3 Method Information F1 Use F1 key to switch between the compact and the detailed list for the method selection Example Line 1 Method number Method name Line 2 Range Line 3 Kind of reagent Line 4 Vial Lines 5 7 Reagents required Tube reagent vial contained in tube test 100 Chlorine 0 01 6 mg l Cl2 tablet 24 mm DPD No 1 DPD No 3 20 Acid demand T 35 Alkali...

Page 60: ...Line 2 Range with chemical species 1 Line 3 Range with chemical species 2 Line 4 Range with chemical species 3 2 3 5 Differentiation Differentiation is possible in some methods e g Chlorine The photometer then requires the type of determination a Press arrow key or to select the required determination Confirm with key 320 Phosphate LR T 0 05 4 mg l PO4 0 02 1 3 mg l P 0 04 3 mg l P2O5 Chlorine T d...

Page 61: ... vial as describe in the relevant application note and place the vial in the sample chamber ensuring that the triangular markings on the vial and HT1000 are aligned Press ZERO key The display shows prepare Zero press ZERO Zero accepted press Test press TEST ...

Page 62: ...When the test result is displayed press the arrow key or Example 320 Phosphate LR T 320 Phosphate LR T 320 Phosphate LR T 0 05 4 mg l PO4 0 02 1 3 mg l P 0 04 3 mg l P2O5 1 00 mg l PO4 0 33 mg l P 0 75 mg l P2O5 If the species of a test result is changed the displayed range is adjusted automatically For an already stored result it is not possible to change the chemical species The last displayed c...

Page 63: ...gnment for the Code No is 0 automatically The display shows The test results is then shown again The entire data set is stored with date time Code No method and test result Note The display also shows the number of free data sets If there are fewer than 30 data sets are free the display shows Clear the memory as soon as possible see Chapter 2 4 3 Delete Stored Results If memory capacity is full it...

Page 64: ...ut the test results without saving beforehand Press F3 key The entire data set is printed with date time Code No method and test result Example The test No is an internal number that is set automatically if a test result is stored It appears only on the printed copy 100 Chlorine T 0 02 6 mg l Cl2 Profi Mode no 2012 06 15 19 30 22 Test No 1 Code Nr 007 4 80 mg l Cl2 ...

Page 65: ...firm with TEST key Or press ZERO key to perform a new zero calibration The display shows 2 3 12 Selecting a New Method Press ESC key to return to method selection Or enter the required method number directly e g Shift 1 6 0 for CyA TEST Cyanuric acid Confirm with key Zero accepted prepare Test press TEST prepare Zero press ZERO ...

Page 66: ... list or by entering the corresponding method number directly The display shows e g 430 nm Always carry out zeroing using a filled vial e g deionised water The display shows Confirm with TEST key The display shows e g TIP To ensure complete reaction times the user countdown may be helpful Chapter 2 2 3 User Countdown 900 mAbs 430 nm 2600mAbs 2600mAbs Prepare Zero Press ZERO Zero accepted prepare T...

Page 67: ...2 Print method 23 Print only results of one selected method 23 Printing params 29 Set printing options 24 Storage 30 Display all stored results 25 Stor date 31 Display only results of selected time period 25 Stor code No 32 Display only results of selected code No range 26 Stor method 33 Display only results of one selected method 27 Delete data 34 Delete all stored results 28 Calibration 40 Speci...

Page 68: ...r polynomial or concentration method 46 User m print 67 Print out all data stored with mode 64 and 65 47 User m init 69 Initialise the user method system 64 and 65 48 Langelier 70 Calculate Langelier Saturation Index water balance 48 Temperature 71 Select C or F for Langelier mode 50 LCD contrast 80 Set the display contrast 51 LCD brightness 81 Set the display brightness 51 System Info 91 Informat...

Page 69: ...th key Key Beep Press MODE Shift 1 1 keys Confirm with key The display shows Press Shift 0 keys to switch the key beep off Press Shift 1 keys to switch the key beep on Confirm with key Note In the case of methods with reaction periods the acoustic signal still sounds during the last 10 seconds of the countdown even if the key beep is switched off Language Deutsch English Français Key beep ON 1 OFF...

Page 70: ...onfirming date and time with key the seconds are adjusted to zero automatically Countdown Ensuring Reaction Times Some methods require a reaction period This reaction period is incorporated in the method as standard with the countdown function It is possible to switch the countdown off for all methods Press MODE Shift 1 3 keys Confirm with key The display shows Clock yy mm dd hh mm _ _ _ _ _ _ _ _...

Page 71: ...y reaction period Non compliance with reaction periods leads to incorrect test results Signal Beep Performing a zero of a measurement takes 8 seconds The photometer indicates the end of zeroing or measuring by a short beep Press MODE Shift 1 4 keys Confirm with key The display shows Press Shift 0 keys to switch the signal beep off Press Shift 1 keys to switch the signal beep on Confirm with key No...

Page 72: ...play shows Press for printing out all stored results The display shows e g After printing the photometer goes back to Mode Menu automatically Note It is possible to cancel the entry by pressing ESC All stored data are printed out See Chapter 2 5 Data Printing Print print all data Start cancel ESC Test No ...

Page 73: ... Confirm with key The display shows Enter year month and day for the first day of the required period e g 19 May 2009 Shift 0 9 0 5 1 9 Confirm with key The display shows e g Press key and all stored results in the selected date range are printed Print sorted date from yy mm dd _ _ _ _ _ _ Test No Print sorted date to yy mm dd _ _ _ _ _ _ Print sorted date from 2009 05 14 to 2009 05 19 Start cance...

Page 74: ...shows Enter numeric code number up to 6 places for the first required Code No e g Shift 1 0 Confirm with key The display shows Press key and all stored results in the selected code number range are printed After printing the photometer goes back to mode menu automatically Note It is possible to cancel the entry by pressing ESC If you want to print only results of one code number enter the same cod...

Page 75: ... differentiated methods select the required kind of determination and confirm with key The display shows e g Press key and all stored results of the selected method are printed After printing the photometer goes back to mode menu automatically Note It is possible to cancel the entry by pressing ESC Print 20 Acid demand T 35 Alkalinity p T 30 Alkalinity tot T Test No Print method 20 Acid demand T S...

Page 76: ...rrow key or to select the required baud rate 1200 2400 400 9600 14400 19200 Confirm with key End with ESC key Back to Mode Menu with ESC key Back to method selection with ESC key 2 4 3 Recall Delete Stored Results Recall All Stored Results Press MODE Shift 3 0 keys Printing params 2 Baud rate cancel ESC Baud rate is 9600 select save cancel ESC ...

Page 77: ...Press arrow key to display the next test result Press arrow key to display the previous test result If there are no test results in the memory the display shows Recall Results of a Selected Time Period Press MODE Shift 3 1 keys Confirm with key The display shows Enter year month and day for the first day of the required period e g 14 May 2009 Shift 0 9 0 5 1 4 Storage display all data Start cancel...

Page 78: ...cted date range are displayed Press F3 key to print the displayed result Press F2 key to print all results End with ESC key Note It is possible to cancel the entry by pressing ESC If you want to recall only results from one day enter the same date twice to determine the time period Storage sorted date to yy mm dd _ _ _ _ _ _ Storage sorted date from 2009 05 14 to 2009 05 19 Start cancel ESC print ...

Page 79: ...ss key and all stored results in the selected date range are displayed Press F3 key to print the displayed result Press F2 key to print all results End with ESC key Note It is possible to cancel the entry by pressing ESC If you want to recall only results of one code number enter the same code number twice If you want to recall all results without code no Code no is 0 enter zero 0 twice Storage so...

Page 80: ... In case of differentiated methods select the required kind of determination and confirm with key The display shows e g Press key and all stored results of the selected method are printed Press F3 key to print the displayed result Press F2 key to print all results End with ESC key Storage 20 Acid demand T 35 Alkalinity p T 30 Alkalinity tot T Test No Storage method 20 Acid demand T Start cancel ES...

Page 81: ...the data sets in memory Press Shift 1 key to delete all the data sets in memory The display shows Press key to delete Press ESC to cancel without deleting data Note All stored test results are deleted 2 4 4 Calibration Calcium Hardness Method 191 Calibration of a Method Blank Press MODE Shift 4 0 keys Confirm with key Delete data Delete all data YES 1 NO 0 Delete data Delete data Do not delete ESC...

Page 82: ...of water crush the tablets using a clean stirring road and dissolve the tablets completely 7 Add 10 CALCIO H No 2 tablets straight from the foil to the same water crush the tablets using a clean stirring rod and dissolve the tablets completely 8 Press key The display shows Wait for a reaction period of 2 minutes The display shows 9 Rinse the vial 24 mm Ø with the coloured sample from the beaker an...

Page 83: ...DTA Preparation Add 50 mg spatula tip full of EDTA to 100 ml of water and dissolve 4 To achieve the most accurate method blank it is important to adhere exactly to the sample volume of 100 ml Calcium Hardness Method 191 Reset Method Blank to Factory Calibration Press MODE Shift 4 0 keys Confirm with key The display shows Press Shift 2 keys The display shows Press Shift 0 keys to keep the method bl...

Page 84: ...rument are aligned 3 Press ZERO key The display shows 4 Remove the vial from the sample chamber 5 Add exactly 2 ml SPADNS reagent solution to the water sample Caution vial is filled up to the top 6 Close the vial rightly with the cap and swirl gently several times to mix the contents 7 Place the vial in the sample chamber making sure that the triangular marks on the vial and instrument are aligned...

Page 85: ...on Press Shift 1 7 0 to select fluoride method Confirm with key If an error message appears please repeat adjustment Notes 1 The same batch of SPADNS reagent solution must be used for adjustment and test The adjustment process needs to be performed for each new batch of SPADNS reagent solution see Standard methods 20th 1998 APHA AWWA WEF 4500 F D S 4 82 2 As the test result is highly the dependent...

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Page 87: ...her test procedure Return to factory calibration If the user calibration has deleted the factory calibration is automatically activated Remarks The fluoride method cannot be calibrated with mode 45 since the test requires a calibration related to the batch of liquid reagent SPADNS mode 40 chapter Fluoride Method 170 see above No Method Recommended range for user calibration 35 Alkalinity p 100 300...

Page 88: ...ybdate 5 15 mg l Mo 257 Nickel 6 8 m l Ni 260 Nitrate LR 0 5 0 7 mg l N 270 Nitrate 0 2 0 3 mg l N 290 Oxygen active Calibration with basic test 100 chlorine free 300 Ozone DPD Calibration with basic test 100 chlorine free 320 Phosphate LR 1 3mg l PO4 321 Phosphate HR 30 50 mg l PO4 329 pH value LR 6 0 6 6 330 pH value 7 6 8 0 332 pH value HR 8 6 9 0 340 Potassium 3 mg l K 350 Silica 0 5 1 5 mg l ...

Page 89: ...ay shows Pressing the arrow key once increases the displayed result Pressing the arrow key once decreases the displayed result Press keys until the displayed result corresponds to the value of the standard Confirm with key Cancel user calibration by pressing ESC key The display shows 100 Chlorine T 0 02 6 mg l Cl2 0 90 mg l free Cl2 User calibration 100 Chlorine T 0 02 6 mg l Cl2 0 90 mg l free Cl...

Page 90: ...zeroing the instrument press MODE Shift 4 6 Confirm with key The display shows Press Shift 1 keys to delete user calibration Press Shift 0 keys to keep the valid user calibration The instrument goes back to zero query automatically 100 Chlorine T 0 02 6 mg l Cl2 1 00 mg l free Cl2 100 Chlorine T 0 02 6 mg l Cl2 Prepare Zero press ZERO User calibration 100 Chlorine T 0 02 6 mg l Cl2 1 00 mg l free ...

Page 91: ...f the Profi Mode is active the photometer provides only a minimum of operator instructions The criteria specified above in d e and f are no longer included Press MODE Shift 5 0 keys Confirm with key The display shows Press Shift 0 keys to switch the Profi Mode off Press Shift q keys to switch the Profi Mode on The display shows either Or Confirm with key Note Storage of test results is possible Wh...

Page 92: ...straight away When the instrument is first being used for an OTZ compatible method and One Time Zero is activated the instrument will request a new Zero with prepare OT Zero Perform Zero as described in the method This Zero will be stored and used for all methods with OTZ function until the instrument is switched off If necessary a new Zero can be performed by pressing ZERO key at any time Press M...

Page 93: ...ive switched on method For this reason it is necessary to activate first all require methods and then to switch off the automatically activated one if this method is not required User Method List Adaptation Press MODE Shift 6 0 keys Confirm with key The display shows Start with key The complete method list is displayed Method with a point behind the method number will be displayed in the method se...

Page 94: ...Cancel without storing by pressing ESC key Recommendation If only a few methods are required it is recommended to perform Mode 62 first followed by Mode 60 All user Polynomials 1 25 and Concentrations 1 10 are displayed in the method list although they are not programmed by the user Non programmed user methods cannot be activated ...

Page 95: ...ft 0 keys to keep the valid method selection The instrument goes back to mode menu automatically User Method List Switch All Methods Off The programme structure requires that the method list must have at least one active switched on method For this reason the instrument activates one method automatically Press MODE Shift 6 2 keys Confirm with key The display shows Press Shift 1 keys to display onl...

Page 96: ...errange and overrrange is defined with 2600 mAbs and 2600 mAbs After selection of a method the concentration of the lowest and highest used standard is displayed as measuring ranger The operation range should be within this range to achieve best results 1000 mAbs 1 Abs 1 E displayed Entering a User Concentration Press MODE Shift 6 4 keys Confirm with key ENTRY PROCEDURE The display shows Enter a m...

Page 97: ...red unit Confirm with key The display shows Press the appropriate numerical key to select the required resolution e g Shift 3 for 0 01 Note Please enter the required resolution according to the instrument pre sets Range Max resolutions User concentr wavelength 1 530 nm 4 430 nm 2 560 nm 5 580 nm 3 610 nm 6 660 nm User concentr choose unit mg l g l mmol l mAbs µg l E A User concentr choose resoluti...

Page 98: ...d water or reagent blank value The display shows Enter the concentration of the first standard e g Shift 0 0 5 Press ESC to delete a digit Press F1 key to reset numerical input Confirm with key The display shows Prepare the first standard and press TEST key User concentr prepare Zero press ZERO User concentr Zero accepted S1 _______ ESC F1 User concentr S1 0 05 mg l prepare Test press TEST ...

Page 99: ...lete a digit Press F1 key to reset numerical input Confirm with key The display shows Prepare the second standard and press TEST key The display shows the input value and measured absorption value Confirm with key User concentr S1 0 05 mg l mAbs 12 User concentr S1 accepted S2 _____ ESC F1 User concentr S2 0 10 mg l prepare Test press TEST User concentr S1 0 10 mg l mAbs 150 ...

Page 100: ...tage e g changing the battery all concentration data will lost and must be entered again You might want to use mode 67 to transfer all concentration data to a PC User Polynomials It is possible to enter and store up to 25 User Polynomials The programme allows the user to apply a Polynomial up to the 5th degree y A Bx Cx2 Dx3 Ex4 Fx5 If only a Polynomial of a lower degree is necessary the other coe...

Page 101: ...no query Press Shift 1 keys to start entry mode The display shows Enter the required wavelength e g Shift 2 for 560 nm The display shows Press arrow key or to change between plus and minus sign Enter data of the coefficient A including decimal point e g Shift 1 3 2 User polynoms Overwrite polynom YES 1 NO 0 User polynoms wavelength 1 530 nm 4 430 nm 2 560 nm 5 580 nm 3 610 nm 6 660 nm User polynom...

Page 102: ... zero 0 is entered for the value of the coefficient the input of the exponent is omitted automatically Confirm every input with key The display shows Enter measurement ranges from 2600 mAbs to 2600 mAbs Press arrow key or to change between plus and minus sign Enter the values in Absorbance mAbs for the upper limit Max and the lower limit Min User polynoms A 1 32___ E ____ User polynoms y A Bx Cx2 ...

Page 103: ...e instrument and can be recalled by entering its method number or selecting it from the displayed method list TIP Save all your polynomial data in a written form because in case of power outage e g changing the battery all polynomial data will be lost and must be entered again You might want to use Mode 67 to transfer all polynomial data to a PC Delete User Methods Polynomial or Concentration In p...

Page 104: ...e selected used method Press Shift 0 keys to keep the valid user method The instrument goes back to mode menu automatically Print Data of User Methods Polynomials Concentration With this mode function all data e g wavelength unit of stored user polynomials and concentration methods can be printed out or transferred with HyperTerminal to a PC Press MODE Shift 6 7 keys User m clear choose no ______ ...

Page 105: ...er loss will cause incoherent data The user method system must be initialised with this mode function to set it to a predefined state ATTENTION All stored user methods polynomial concentration are deleted with initialisation Press MODE Shift 6 9 keys Confirm with key The display shows Confirm with key The display shows the query Press Shift 1 keys to start initialisation User m print Start User m ...

Page 106: ... the Langelier Saturation Index With mode 71 see below it is possible to select between degree Celsius and degrees Fahrenheit Press MODE Shift 7 0 keys Confirm with key The display shows Enter the temperature value T in the range between 3 and 53 C and confirm with key If F is selected enter the temperature value in the range between 37 and 128 F The display shows Enter the value for calcium hardn...

Page 107: ... the pH value in the range between 0 and 12 and confirm with key The display shows the Langelier Saturation Index Press key to start new calculation Return to mode menu by pressing ESC key Operating error Examples Values out of defined range The entered value is too high Langelier Tot alkalinity 5 TA 800 _ _ _ _ _ Langelier Total dissol solids 0 TDS 6000 _ _ _ _ _ Langelier pH value 0 PH 12 _ _ _ ...

Page 108: ...value is possible in degrees Celsius or degrees Fahrenheit Therefore the following preselection is once required Press MODE Shift 7 1 keys Confirm with key The display shows Press Shift 1 keys to select degrees Celsius Press Shift 2 keys to select degrees Fahrenheit The instrument goes back to mode menu automatically CH 1000 mg l CaCO3 temperature 1 C 2 F ...

Page 109: ...he LCD display by one unit Press arrow key to decrease the contrast of the LCD display by one unit Press STORE key to increase contrast of the LCD display by 10 units Press TEST key to decrease the contrast of the LCD display by 10 units Confirm with key Adjusting Display Contrast Press MODE Shift 8 1 keys Confirm with key LCD contrast 1 1 Store Test 10 10 0 254 180 ...

Page 110: ... one unit Press arrow key to decrease the contrast of the LCD display by one unit Press STORE key to increase contrast of the LCD display by 10 units Press TEST key to decrease the contrast of the LCD display by 10 units Confirm with key LCD brightness 1 1 Store Test 10 10 0 254 60 ...

Page 111: ...a or to transmit to a PC the optional Trace2o PC Link infra red module is required 2 5 1 Data Printing Besides the PC Link module the following printer is required to print data directly using the USB Interface of the module HP Deskjet 6940 2 5 2 Data Transfer to a Personal Computer Besides the PC Link a transfer programme is required to transmit test results Please find detailed information in th...

Page 112: ...acklight Serial Interface IR interface for data transfer Light Source Light emitting diode photosensor pair arrangement in a transparent measurement chamber Wavelength ranges λ1 530 nm IF Δ λ 5 nm λ2 560 nm IF Δ λ 5 nm λ3 610 nm IF Δ λ 6 nm λ4 430 nm IF Δ λ 5 nm λ5 580 nm IF Δ λ 5 nm λ6 660 nm IF Δ λ 5 nm IF Interference filter Wavelength Accuracy 1 nm Photometric Accuracy 2 FS T 20 C 25 C Photome...

Page 113: ...s Approx 210 x 95 x 45 mm unit Approx 395 x 295 x 106 mm case Weight unit Approx 450 g Working Condition 5 40 C at max 30 90 relative humidity without condensation Language Options English German French Spanish Italian Portuguese Polish further languages possible upon request Storage Capacity Ca 1000 data sets measured with standard solutions SUBJECT TO TECHNICAL MODIFICATION To ensure maximum acc...

Page 114: ...rdness Abs Absorption unit Extinction E 1000 mAbs 1 Abs 1 A 1 E µg l ppb Microgram per litre mg l ppm Milligram per litre g l ppth Gram per litre KI Potassium Iodide KS 4 3 Acid demand to pH 4 3 this method is similar to total alkalinity but converted to the unit mmol l as the German IN 38409 demand TDS Total Dissolved Solids LR Low Range MR Medium Range HR High Range L Liquid Reagent P Powder rea...

Page 115: ...N N Diethylhydroxylamine DPD Diethyl p phenylenediamine DTNB Ellman s reagent PAN 1 2 Pyridylazo 2 napthol PMAB Paradimethylaminobenzaldehyde PPST 3 2 Pyridyl 5 6 bis 4 phenylsulfonic acid 1 2 4 triazine TPTZ 2 4 6 Tri 92 Pyridyl 1 3 5 triazine ...

Page 116: ... sample is too cloudy Filter water sample Too much light on the photo cell Seal the cap Repeat measurement with seal on the cap of the vial Underrange Results is under the detection limit Indicate results with lower x mg l X low end of measuring range if necessary use other analytical method Storage system error Use mode 34 Mains power fails or is not connected Insert or change battery Delete data...

Page 117: ...ser calibration Jus Underrange E4 Overrange E1 The concentration of the standard is too high low during the user calibration the limit of the range was exceeded Perform the test with a standard of higher lower concentration Underrange E1 E40 User calibration not possible If the display shows overrange underrange for a test result a user calibration is not possible Perform the test with a standard ...

Page 118: ...ot repeat test Example 1 Example 1 The readings for free and total chlorine are different but considering the tolerances of each reading they are the same For this reason the combined chlorine is most likely zero Example 2 Example 2 The reading for free chlorine is under the detection limit The instrument is 0 6 mg l free Cl comb Cl 0 59 mg l total Cl Underrange comb Cl 1 59 mg l total Cl ...

Page 119: ... chlorine is most likely the same as total chlorine Example 3 Example 3 The reading for total chlorine is exceeding the range the instrument is not able to calculate the combine chlorine The test should be repeated with a diluted sample 0 6 mg l free Cl comb Cl Overrange ...

Page 120: ...de off with mode 50 The pre programmed countdown is not displayed Countdown is not activated and or the Profi Mode is activated Switch the countdown on with mode 13 and or switch the Profi Mode off with mode 50 It seems that a method is not available Method is not activated in the user method list Activate the required method in the user method list with mode 60 Trace2o Ltd Technology Centre Wagte...

Page 121: ...tion of Ammonia P60 using the HT1000 Photometer Equipment HT1000 Photometer 10ml vial Stirring rod HT9 Ammonia No 1 tablet HT10 Ammonia No 2 tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used HydroTest reagents before the formulation may have changed Getting started ...

Page 122: ...ft followed by 60 Release shift and press enter Blank analysis Ensure that the 10ml vial is clean Fill the vial with 10ml of the water sample Fit the cap and tighten Place the vial in the sample chamber making sure that the arrow marks on the instrument and vial are aligned Press the zero key Wait until the following is displayed Zero accepted Prepare Test Press TEST ...

Page 123: ...lean stirring rod until no large pieces are visible Close the vial tightly with the cap and swirl several times until the tablet is dissolved Analysis Place the vial in the sample chamber making sure that the marks on the instrument and vial are aligned Press the Test key Wait for a reaction period of 10 minutes After the reaction period the measurement starts and the result is displayed in mg l A...

Page 124: ...le The tablets must be added in the correct sequence Ammonia No 1 tablet will only dissolve fully after Ammonia No 2 tablet is added The tablets should be added direct from the foil avoiding contact with hands or surfaces The temperature is critical for full sample development Below 20 C increase reaction time to 15 minutes The tablets are unsuitable for determination of ammonia in sea water or br...

Page 125: ...otal Chlorine P100 using the HT1000 Photometer Equipment HT1000 Photometer 10ml vial Stirring rod HT15 DPD No 1 tablet HT16 DPD No 3 tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used HydroTest reagents before the formulation may have changed Getting started Switch the unit on using the power key ...

Page 126: ...til the double arrow is adjacent to the required test diff free total and press enter Blank analysis Ensure that the 10ml vial is clean Fill the vial with 10ml of the water sample Fit the cap and tighten Place the vial in the sample chamber making sure that the arrow marks on the instrument and vial are aligned Press the zero key Wait until the following is displayed Zero accepted Prepare Test Pre...

Page 127: ...e cap and swirl several times until the tablet is dissolved Analysis Free Chlorine Place the vial in the sample chamber making sure that the marks on the instrument and vial are aligned Press the Test key Wait for a few seconds until the result is displayed in mg l free Chlorine Sample preparation Total Chlorine Add one HT15 DPD No 1 tablet and one HT16 DPD No 3 tablet straight from the foil to th...

Page 128: ...ligned Press the Test key Wait for a reaction period of 2 minutes After the reaction period the measurement starts and the result is displayed in mg l total Chlorine Sample preparation Differentiated Determination Add one HT15 DPD No 1 tablet straight from the foil to the water sample Crush the tablet using a clean stirring rod until no large pieces are visible Close the vial tightly with the cap ...

Page 129: ...e visible Close the vial tightly with the cap and swirl several times until the tablet is dissolved Place the vial in the sample chamber making sure that the marks on the instrument and vial are aligned Press the Test key Wait for a reaction period of 2 minutes After the reaction period the measurement starts and the result is displayed in mg l free Chlorine mg l combined Chlorine mg l total Chlor...

Page 130: ...ould be added direct from the foil avoiding contact with hands or surfaces The sample should have a pH value of between 6 2 and 6 5 after addition of the reagent High Calcium ion content and or high conductivity can lead to turbidity of the sample and therefore incorrect measurements a solution is available if necessary Chlorine High Ca reagents can be purchased from your Trace2o distributor and a...

Page 131: ... The following application note explains the procedure for the detection of Nitrate P260 using the HT1000 Photometer Equipment HT1000 Photometer Nitrate test tube Nitrate test powder Nitrate Test tablet 10ml vial Stirring rod Nitrite LR tablet Safety ...

Page 132: ...t reagents before the formulation may have changed Getting started Switch the unit on using the power key Select the Nitrate method by depressing the cursor keys until Nitrate T is displayed or use the shortcut by pressing holding shift followed by 260 Release shift and press enter Blank analysis ...

Page 133: ...the zero key Wait until the following is displayed Sample preparation Fill the nitrate test tube with 20ml of the water sample Add 1 level spoon of Nitrate Test powder Close the tube tightly with the cap and swirl vigorously for one minute Add one Nitrate Test tablet straight from the foil to the water sample Close the tube tightly with the cap and swirl vigorously for one minute Zero accepted Pre...

Page 134: ...of the treated solution into the 10ml vial Add one Nitrite LR tablet straight from the foil to the water sample Crush the tablet using a clean stirring rod until no large pieces are visible Close the vial tightly with the cap and swirl several times until the tablet is dissolved Analysis Place the vial in the sample chamber making sure that the marks on the instrument and vial are aligned Press th...

Page 135: ...ways handle vials by the lid where possible The tablets should be added direct from the foil avoiding contact with hands or surfaces Nitrite present in the sample will also react leading to a higher result To correct carry out a nitrite test P270 on the same sample and subtract the result Nitrate concentrations above 1 mg L can be diluted up to 100x with the result multiplied up to compensate Inte...

Page 136: ......

Page 137: ...O AN P270 NITRITE P270 METHOD The following application note explains the procedure for the detection of Nitrite P270 using the HT1000 Photometer Equipment HT1000 Photometer 10ml vial Stirring rod Nitrite LR tablet Safety ...

Page 138: ...t reagents before the formulation may have changed Getting started Switch the unit on using the power key Select the Nitrite method by depressing the cursor keys until Nitrite T is displayed or use the shortcut by pressing holding shift followed by 270 Release shift and press enter Blank analysis ...

Page 139: ...rument and vial are aligned Press the zero key Wait until the following is displayed Sample preparation Add one Nitrite LR tablet straight from the foil to the water sample Crush the tablet using a clean stirring rod until no large pieces are visible Close the vial tightly with the cap and swirl several times until the tablet is dissolved Analysis Zero accepted Prepare Test Press TEST ...

Page 140: ...e result is displayed in mg l Nitrite LOD Tolerance The Lower LOD is 0 01 mg l 10ppb upper LOD is 0 5 mg l 500ppb Tolerance 0 005mg l Notes For best results rinse vials thoroughly between with deionised water Ensure that the outside of the vials are clean dry and free from fingerprints Always handle vials by the lid where possible The tablets should be added direct from the foil avoiding contact w...

Page 141: ... Interferences may occur from presence of the following ions Antimony III Iron III Lead Mercury I Silver Chloroplatinate Metavanadate Bismuth Copper II ions may artificially lower test results ...

Page 142: ...HydroCheck HC1000 Multi Parameter Electrochemical Water Tester Instruction Manual ...

Page 143: ...lling Data from Memory 108 4 1 Setup Menu 110 4 2 Adjusting the settings 116 4 3 Exiting setup mode 117 4 4 Setting the Date and Time 117 4 5 Temperature Compensation 118 4 6 Automatic Temperature Compensation 118 4 7 Manual Temperature Compensation 119 5 1 pH Calibration 119 5 2 Single Point Calibration 120 5 3 Multi Point Calibration 121 5 4 pH Calibration with User Defined Buffers 122 5 5 pH Ca...

Page 144: ...rode Care and Maintenance 138 11 2 ORP Electrode Care and Maintenance 139 11 3 Conductivity Electrode Care and Maintenance 140 11 4 DO Probe Care and Maintenance 140 12 1Troubleshooting 141 13 1 Appendix 1 How to calculate the temperature coefficient 142 13 2 Appendix 2 How to calculate the TDS conversion factor 143 13 3 Appendix 3 Pressure vs Altitude Table 144 14 1 Warranty 145 ...

Page 145: ...0 Ambient temperature is greater than 0o C and less than 60o C No potential electromagnetic interference The following list describes the standard accessories of the meter After the unpacking please check all accessories are complete If any are damaged or missing please contact nearest distributor 1 3 List of Accessories pH Electrode Conductivity Electrode Dissolved Oxygen Electrode pH Buffer Solu...

Page 146: ...indicator Memory mode Calibration Due Reminder Electrode slope indicator Automatic Temperature Compensation 2 2 Keypad The Trace2o HydroCheck HC1000 has a tactile membrane keypad Words and symbols describe functions of each key The direction keys are multi function left right and up down keys ...

Page 147: ...p through menu Press in temperature setting mode to increase the setting value mR Press to view calibration report or stored data Press in setup mode to scroll down through menu Press in temperature setting mode to decrease the setting value enter Confirms the calibration setting value or displayed option Press and hold to turn On Off the backlight 2 3 Connectors The Trace2o HydroCheck HC1000 has ...

Page 148: ...r on the pH electrode into corresponding connector on the instrument Once pushed firmly into place twist to ensure water tightness Conductivity Electrode Insert the 3 pin connector on the conductivity electrode into the corresponding connector on the instrument ensuring that the lugs are aligned Once pushed firmly into place tighten the waterproof locking collar ...

Page 149: ...alled with the requisite battery The HC1000 requires 1 x 9V PP3 battery To replace the battery follow the below procedure 1 Remove the battery cover from the rear of the meter 2 Disconnect the snap connector and remove the battery 3 Note the polarity and attach the snap connector to the appropriate terminals on the new battery 4 Ensure that the white waterproofing seal is correctly located and rep...

Page 150: ...rew the membrane cap from the bottom of the dissolved oxygen probe 2 Fill the membrane cap halfway with electrolyte solution 3 Screw the membrane cap onto the probe excess electrolyte will drain out 4 Be sure the cathode of probe makes contact with membrane cap the electrolyte solution in membrane cap should be without an air bubble Prior to Use Remove the protective cap from the bottom of the sen...

Page 151: ...tor 2 Rinse the pH electrode with distilled water to clean 3 Immerse the electrode in the sample solution and stir the solution gently 4 Wait for the reading to stabilise and record the value shown on the display 3 3 ORP Measurement The HC1000 has two millivolt measurement modes ABSOLUTE MILLIVOLT MODE Press the MODE key until the display shows the measurement unit mV the meter enters absolute mil...

Page 152: ...Select one of the above modes Immerse the electrode in the sample solution and stir the solution gently Wait for the reading to stabilise and record the value shown on the display ...

Page 153: ...asurement The HC1000 has two salinity measurement modes practical salinity unit PSU and natural seawater unit ppt 1 Press the MODE key until the display shows the indicator and measurement unit PSU the meter enters practical salinity measurement mode 2 If necessary press the MODE key again the display shows the indicator and measurement unit ppt the meter enters seawater measurement mode 3 Select ...

Page 154: ...l influence the measurement of dissolved oxygen Clean samples are preferred If the sample contains solvent grease sulfide and algae the membrane on the probe may be blocked damaged or eroded 1 Press MODE key until the meter shows indicator 2 Connecting the dissolved oxygen probe to meter wait for 15 minutes to polarise the sensor 3 If necessary to set the atmospheric pressure and salinity coeffici...

Page 155: ...ently displayed value Press the ON OFF key again to resume measuring 3 7 Storing and Recalling Data from Memory The HC1000 allows up to 500 data sets to be stored and recalled MEMORY INPUT During the measurement process press the M key to store the measured value to the memory The Memory indicator appears on the display MEMORY RECALL 1 Press MR key in the measurement mode the meter shows LOC P 01 ...

Page 156: ... key the meter shows the date and time of the reading Format mm dd hh mm 4 Press the key again the display shows the stored data 5 After viewing the memory press the ON OFF key to return to measurement mode MR MR ...

Page 157: ...lied to all modes pH MODE DISPLAY DESCRIPTION OPTION S DESCRIPTION DEFAUL T Select from common pH buffer standard groups for calibration USA 1 68 4 01 7 00 10 01 12 45 NIST 1 68 4 01 6 96 9 18 12 45 DIN 1 09 4 65 6 79 9 23 12 75 User Defined Buffer Select the number of calibration points to use when calibrating the meter 1 point 2 points 3 points 4 points 5 points Set the resolution for pH measure...

Page 158: ...ductivity electrode K 0 1 K 1 K 10 User Defined Select the temperature coefficient of sample Setting Range 0 0 to 10 0 o C 2 10 Select the number of calibration points to use when calibrating the meter 1 point 2 points 3 points 4 points 5 points Pure water coefficient will be calculated and applied automatically for ultra pure water measurement if enabled Enable Disable Set the normalization tempe...

Page 159: ...measurement and calibration Set the default TDS conversion factor Setting Range 0 40 to 1 00 0 5 Set the default temperature unit Degrees Celsius Degrees Fahrenheit ...

Page 160: ...eric pressure coefficient Setting Range 450 to 850mmHg 760 Select the salinity coefficient of sample solution Setting Range 0 0 to 50 0ppt 0 0 Set the resolution for DO measurement 0 01mg L 0 1 0 1mg L 1 Set the default measurement units Degrees Celsius Degrees Fahrenheit mg L Milligrams per litre ppm Parts per million mmHg Pressure unit kPa Pressure unit ...

Page 161: ...urate When the HI option is enabled measuring value will stabilize slowly but improves accuracy Low High When the auto hold function is enabled the meter will automatically sense a stable end point reading and hold Enable Disable When the auto off power is enabled the meter will turn off automatically if there are no key presses within the specified time period 10 minutes 20 minutes 30 minutes Dis...

Page 162: ...e meter will automatically show indicator 1 to 31 days Disable Sets the date and time of the meter Clear all stored data Enable Disable Reset function allows user to restore the meter to factory default settings When this function is used all calibration values and settings will be lost or reset Enable Disable ...

Page 163: ...CAL key for 3 seconds the meter enters setup mode the display shows adjustable setting and parameter number 3 Press or key to scroll through the menu and select the parameter to amend Refer to Setup Menu section 4 Press ENTER key to confirm the display shows the currently selected option MI Enter Setting Parameter number Setting Current Option ...

Page 164: ...urn to measurement mode immediately 4 4 Setting the Date and Time The HC1000 has a real time clock that is used to time stamp stored measurement values and calibration data The below instructions describe initial setup of the date and time values 1 Press and hold the CAL key for 3 seconds to enter setup mode 2 Press or key until the display shows the DATE option 3 Press ENTER key to confirm the me...

Page 165: ...rement mode 4 5 Temperature Compensation For accurate results either manual or automatic temperature compensation must be enabled before measurement or calibration 4 6 Automatic Temperature Compensation Insert the 6 pin connector on the temperature probe into the corresponding connector on the instrument ensuring that the lugs are aligned Once pushed firmly into place tighten the waterproof lockin...

Page 166: ...e pressing and releasing the or key will increase or decrease the temperature value by 0 1 Pressing and holding the or key will increase or decrease the temperature value by 1 5 1 pH Calibration The HC1000 allows up to a 5 point calibration in the pH mode We recommend performing at least a 2 point calibration for more accurate measurement The meter will automatically recognise and calibrate to fol...

Page 167: ...he measurement 5 2 Single Point Calibration 5 2 1 Ensure that 1 point calibration is selected in the setup menu 5 2 2 Rinse the pH electrode with distilled water 5 2 3 Ensure the display shows the pH indicator and press CAL key the meter shows pH7 00 CAL or 6 86 CAL 6 79 CAL 5 2 4 Immerse the pH electrode in the pH7 00 or 6 86 or 6 79 buffer solution The tip of the electrode must be completely sub...

Page 168: ...3 Rinse the pH electrode with distilled water Dip the electrode into the next buffer solution e g pH4 01 The meter automatically senses the current calibration solution and begins calibrating Calibration indicator begins flashing 5 3 4 Wait for the measured value to stabilise the display shows CAL3 The meter prompts to continue with third calibration point 5 3 5 Repeat the steps from 5 3 3 until t...

Page 169: ...ontinue with second calibration point 5 4 5 Rinse the pH electrode with distilled water Immerse the electrode in the next custom buffer solution 5 4 6 Press or key to increase or decrease displayed value according to the value of the custom buffer and press ENTER key to confirm Calibration indicator begins flashing 5 4 7 Wait for the measured value to stabilise the display shows END Once calibrati...

Page 170: ...s ELE P 02 Electrode Diagnosis 5 5 3 Press ENTER key to confirm the meter shows the last calibration date Format mm dd 5 5 4 Press key the meter shows OFS zero point offset potential 5 5 5 Press key again the meter shows the slope of each pH buffer group 5 5 6 Press the ON OFF key to return to measurement mode If the meter is not calibrated or calibration is not successful the display will show on...

Page 171: ...ws indicator 2 Rinse the ORP electrode with distilled water Immerse the electrode in the calibration solution Stir the solution gently 3 Press CAL key the meter shows the current measured value 4 Press or key to set the displayed value press ENTER key to confirm The Calibration indicator begins flashing 5 Wait for the measured value to stabilise the display shows END Once calibration is complete t...

Page 172: ...mR key in the ORP measurement mode the display shows LOC P 01 2 Press or key until the meter shows ELE P 02 Electrode Diagnosis 3 Press ENTER key to confirm the meter shows the last calibration date Format mm dd 4 Press key the meter shows OFS Offset Potential 5 Press the ON OFF key to return to measurement mode MR Enter MR ...

Page 173: ...s recommended Alternatively it is advised to select a standard value close to the sample value under measurement The meter will automatically detect common conductivity standard solutions and prompt the user to calibrate the meter When the calibration is completed all new calibration values will automatically override existing data The following table shows acceptable conductivity range of calibra...

Page 174: ...e CAL key the meter enters the calibration mode 7 2 4 Immerse the conductivity electrode in the calibration solution The meter automatically shows the detected calibration standard e g 1413µS cm 7 2 5 Press ENTER key to confirm Calibration indicator begins flashing 7 2 6 Wait for the measured value to stabilise the display shows END Once calibration is complete the meter returns to measurement mod...

Page 175: ...de with distilled water Immerse the electrode in the next calibration solution the meter automatically shows the currently detected calibration standard e g 12 88mS cm 7 3 4 Press ENTER key to confirm Calibration indicator begins flashing 7 3 5 Wait for the measured value to stabilise the display shows CAL3 The meter prompts you to continue with third point calibration 7 3 6 Repeat steps 2 3 to 2 ...

Page 176: ...a label attached to the electrode e g K 1 08 7 4 2 Ensure the Cal indicator is showing and press and hold the CAL key for 3 seconds to enter setup menu the display shows CELL option 7 4 3 Press ENTER key to confirm the meter enters the cell constant setting mode 7 4 4 Press or key to select USER option 7 4 5 Press ENTER key to confirm the meter enters User Defined mode Enter MI Enter ...

Page 177: ... immediately Performing the conductivity calibration will simultaneously calibrate the corresponding TDS salinity and resistivity value 7 5 Conductivity Calibration Report The meter allows checking of the calibration factor of the conductivity electrode for diagnostic purposes 1 Press the MR key in the conductivity measurement mode the display shows LOC P 01 2 Press or key until the meter shows EL...

Page 178: ...mediately 8 1 DO Calibration in Saturation Mode The HC1000 dissolved oxygen probe can be calibrated quickly in air In the percentage saturation mode the meter is able to perform either 1 or 2 points calibration For single point calibration it is recommended to perform 100 saturation calibration in air For the 2 point calibration the second point should be saturated anhydrous sodium sulfite solutio...

Page 179: ...ibration is selected in the setup menu 8 2 3 Press CAL key the meter enters calibration mode the display shows 100 CAL1 8 2 4 Hold the dissolved oxygen probe in the air press ENTER key to confirm Wait for the measured value to stabilise the display shows END Once calibration is complete the meter returns to measurement mode automatically ...

Page 180: ...urated anhydrous sodium sulfite solution zero oxygen solution Stir the probe gently 8 3 5 Press ENTER key to confirm Calibration indicator begins flashing 8 3 6 Wait for the measured value to stabilise the display shows 100 CAL2 The meter prompts you to continue with second point calibration 8 3 7 Immerse the dissolved oxygen probe in air saturated water for 3 to 5 minutes press ENTER key to confi...

Page 181: ...te the meter returns to measurement mode automatically N B Performing a percentage saturation calibration will simultaneously calibrate the corresponding mg L or ppm concentration value Therefore additional mg L calibration isn t required in most circumstances ...

Page 182: ...pheric pressure coefficient in the setup menu Refer to Setup Menu section 9 1 4 Press CAL key the display shows 8 25mg L CAL1 25o C 9 1 5 Dip the dissolved oxygen probe into the air saturated water for 3 to 5 minutes 9 1 6 Press ENTER key to confirm Calibration indicator begins flashing 9 1 7 Wait for the measured value to stabilise the display shows END Once calibration is complete the meter retu...

Page 183: ... oxygen solution Stir the solution gently 9 2 5 Press ENTER key to confirm Calibration indicator begins flashing 9 2 6 Wait for the measured value to stabilise the display shows 8 25 CAL2 The meter prompts you to continue with second point calibration 9 2 7 Immerse the dissolved oxygen probe in air saturated water for 3 to 5 minutes press ENTER key to confirm Wait for the measured value to stabili...

Page 184: ...c temperature compensation is enabled the meter will need to be calibrated if the temperature reading displayed differs from that of an accurate thermometer 10 1 1 Press and hold the MODE key for 3 seconds to enter temperature calibration mode the display shows current temperature reading 10 1 2 Press or key to set the temperature value 10 1 3 Press ENTER key to confirm Calibration is completed ...

Page 185: ... minutes Clogged reference junction Heat a diluted KCl solution to 60 o C to 80o C Place the tip of the electrode into the heated solution for about 10 minutes Allow the electrode to cool in some room temperature KCl solution Protein deposits Prepare a 1 pepsin solution in 0 1M of HCL Place the electrode in the solution for 10 minutes Rinse the sensor with distilled water REACTIVATING THE ELECTROD...

Page 186: ...ctrode storage solution CLEANING THE ELECTRODE Contamination of the sensor often results in slow response and inaccurate readings If necessary clean the element by one of the following procedures Inorganic Deposits Soak the ORP electrode in 0 1M HCl for 10 minutes Remove and rinse with distilled water then place in isopropyl alcohol for 5 minutes Remove and rinse again and soak in pH 4 01 buffer s...

Page 187: ...moved very carefully with a cotton bud soaked in solvent taking care not to touch the metal parts of the inner cell 11 4 DO Probe Care and Maintenance Always keep probe membrane moist If dissolved oxygen probe is not in use for long periods unscrew membrane cap and rinse the probe s cathode anode and the membrane with deionised water then soak up residual water with filter paper and re install the...

Page 188: ...whether clogged dirty or broken Incorrect calibration solutions Using the fresh calibration solutions for calibration Setting value does not match calibration solution Reset the calibration value pH electrode is out of service life Replace the pH electrode Keypad is not working properly Replace the batteries DO electrolyte solution is depleted Refill the electrolyte solution Zero oxygen solution i...

Page 189: ... hold the MODE key for 3 seconds to enter temperature setting mode 2 Press or key to set the temperature at 25o C 3 Dip the conductivity electrode and temperature probe into sample solution A 4 Record the temperature value TA and conductivity value CTA 5 Repeat the steps above whilst dipping the electrode and probe into same sample solution B Make sure both sample solutions have about 5o C to 10o ...

Page 190: ...ermine the TDS conversion factor use the following formula Factor Where Actual TDS value of a precisely weighed quantity of anhydrous NaCl or KCl dissolved in high purity water Actual Conductivity the meter measured conductivity value For example Dissolve 64 grams of potassium chloride reagent in 1L distilled water If its conductivity value is 100mS cm then TDS conversion factor is 0 64 Actual TDS...

Page 191: ...60 100 100 1 750 200 98 8 741 300 97 6 732 400 96 4 723 500 95 2 714 600 94 0 705 700 92 8 696 800 91 7 688 900 90 5 679 1000 89 4 671 1100 88 3 662 1200 87 2 654 1300 86 1 646 1400 85 0 638 1500 84 0 630 1600 82 9 622 1700 81 9 614 1800 80 9 607 1900 79 9 599 2000 78 9 592 ...

Page 192: ...ed by the Trace2o Ltd standard warranty terms and conditions available via email or via download from www trace2o com In the event that any technical assistance is required Trace2o Customer service department will be happy to assist Contact details as follows Trace2o Ltd The Technology Centre Station Road Thatcham Berkshire RG19 4HZ UK T 01635 866772 E Technical Trace2o com ...

Page 193: ...NOTES ...

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