Abaxis VetScan HM5, Operator'S Manual

Page: 95 / 164

Troubleshooting 9-7
L
Insufficient Lyse reagent
delivered to burst all
RBCs (or lyse-resistant
RBCs), platelet clumping
or large platelets for a
Cavalier King Charles
Spaniel. • If patient is a Cavalier King Charles Spaniel or other breed
with known predisposition to macro-platelets, perform a
manual smear.
• If the draw was difficult, the platelets are low, or there were
delays in filling the tube, redraw and rerun.
• Make sure the Lyse tube (yellow) has no kinks, or bends and
that nothing is compressing the tubing.
• Check for bubbles in the Lyse tube. If found, prime the Lyse:
see “Check Tubing” on page 4-4 .
• If no Lyse issues are found, rerun the sample with increased
Lyse +0.1 ml, then if needed with +0.2 ml: see “Adjusting the
Lyse Volume” on page 4-9 .
• If the L Flag still persists, draw a new sample, aiming for a
clean needle puncture and fast transfer to the EDTA tube.
• If the
L
flag still persists, call Abaxis Technical Support.
W
Severe platelet clumping
or very large WBCs. • If the cat platelet his-
togram resembles the
diagram at right,
redraw the sample,
reduce delays in fill-
ing the tube, and mix
well with at least 10-15 inversions immediately.
• If the
W
flag persists, call Abaxis Technical Support.
Y
• Reagent tubing con-
nected to incorrect
port or bottle, clogging,
or insufficient Lyse 2
delivered.
• If the
Y
flag is accom-
panied by many other
warnings (
c
,
C
,
E
,
B
,
Y
), call Abaxis Techni-
cal Support.
• Make sure the Lyse 2 tube (orange) is connected to the
Lyse 2 bottle. If not, disconnect the incorrectly connected
tubes from the analyzer, and hold them up so the liquid in
them flows back into the bottles. Connect tubes to the cor-
rect connections on the analyzer, bottle caps or dip tubes by
color-matching the tubes, analyzer port, and bottle caps or
dip tube heads, then prime Lyse 2.
• If the tubing is already correctly connected, check the Lyse 2
tubing connections at the analyzer and the bottle cap/dip
head. Make sure the tube is securely connected to the con-
nector. If this reagent pack was newly installed, prime the
Lyse 2 twice, then rerun the sample.
• If the Lyse 2 tube is securely connected, run 1-3 automatic
self-cleanings (see “Automatic Self-Cleaning” on page 6-8 ),
then rerun the sample or blank that gave the
Y
warning.
• If the
Y
flag persists, call Abaxis Technical Support.
Z
Lyse 2 delivery issues, or
a dirty Wash Head or
aperture. • Check the Lyse 2 tube as described above for the
Y
flag.
• If the Lyse 2 tube connected correctly, inspect the Wash
Head and clean as needed (see “Cleaning the Wash Head”
on page 6-4 ), then run 1-3 automatic self-cleanings (see
“Automatic Self-Cleaning” on page 6-8 ). Rerun the sample.
• If the
Z
flag persists, call Abaxis Technical Support.
Flag Meaning Description / Recommended action