Abaxis VetScan HM5, Operator'S Manual

Page: 138 / 164

C-2 Potential Sample Interferences
T
abl
e C-1: Intrinsi
c Subst
an
ces: Poten
tial Sampl
e
-Induce
d Interference
s
Samp
le Con
d
itio
n
Ind
ic
ator
s
R
e
s
u
lt
s
Affected
Ca
us
es
Solution
s
Lipemia
•
C
loudy
, white plasma.
•
H
igh MCHC.
Increased HGB, MCHC
, MCH. RBCs
may be
sm
udged on
blood film.
Non
-fasted sample
Redraw fa
st
ed
s
ample.
Met
abolic disorder
a
Use RBC, PCV
, MCV
, and RDW ra
ther than
HGB, MCH
, MC
HC values to assess
ane
mia.
Hemolysis
P
ink or red plasma.
•
De
cr
eased RBC,
PCV
•
Increased MCHC
T
rauma
tic
venipuncture
Redraw w/ clean venipuncture. Remove nee
dle
before dispensing blood into tube.
Hemolytic ane
mia
N
/A
Clumped
platelet
s
•
Decreased platelet
count w/
platelet clump
s
of
ten visible on blood smear or applicator
stick
di
pped in s
ample.
•
R
is
ing lef
t side
of ly
mphocyte curve.
•
S
mall p
ale clot
s stick to wooden ap
plicator
stick
swirled in sampl
e.
•
Low platelet histogram with a rising rig
ht
side.
•
P
ossible t
ailing on righ
t s
ide o
f granulocyte
curve.
Dec
reased PL
T
+/- increased WBC
T
rauma
tic
venipuncture or fe
line
species
Redraw; collect blood in anti-c
oa
gulated
syri
nge
or use
vac
ut
ainer system;
in
ve
rt tube
several times immediately af
ter filling; vortex
sample
immediately before testing.
Excess pot
assium
EDT
A
(under-filled
tube)
Fill tub
e at least halfway
, or remove portion
of p
ot
assium EDT
A
before filling tube.
Miscellane
ous/
idi
op
athic
Use altern
ative an
ti-coagulant as hep
arin
or
citrate; vortex sample imme
diat
ely prior to
sampling.
Giant platelet
s
R
ight sid
e of platelet histogram runs in
to RBC
histogram.
Dec
reased PL
T,
decreased MPV
Thrombopoiesis
,
feli
ne
speci
es
Confirm platelet estimate on smear and/or manual platelet count.
Clotted sample
•
V
isible clot in sample.
•
R
ed clot(s) stick to wooden applicator stick
swi
rled in sample.
•
P
latelet clump
s may or may not
be visible
on blood smear
.
Dec
reased PL
T,
decreased WBC, and/or decreased RBC (varies with clot si
ze)
T
rauma
tic
venipuncture and/or delayed transfer to anti-coagulant
Redraw with clean venipuncture
; use
vacut
ai
ne
r system or anti-coagulated
syringe
; mix collection tube b
y multiple
invers
ions immediately af
ter filling. Clotted
sample
may clog the sample needle.
Lyse-resist
ant RBCs
•
R
is
ing lef
t side
of ly
mphocyte curve.
•
L flag in dogs.
Increased WBC, increased L
ymphs
Idiop
athic (most
commonly with feli
ne
s)
Lyse volume
may be adjusted for the sample
(for additional information, cont
act Ab
ax
is
Te
chnical Support — see
page
1-3
).
a).Dia
betes me
llitus, neph
rotic
synd
rome,
h
yp
o
thyroid
ism,
lip
oprotein
li
pa
se
d
efi
ciency
, a
cute p
ancrea
titis,
ch
ole
st
asis,
hyper
a
dreno
co
rti
ci
sm, hyperch
olesterol
emia in
bri
ards, id
iop
a
th
ic h
yperli
pi
de
mi
a of
min
iature schna
uzers.