Abaxis VetScan HM5, Operator'S Manual

Page: 47 / 164

Running a Sample 4-9
4.6 Adjusting the Lyse Volume
The Lyse volume generally only needs adjustment on rare occasions if the patient has Lyse-resistant red
blood cells, as indicated by an
L
warning on the results after other potential causes have been elimi-
nated. For details on other potential causes and instructions, see “Result Warning Flags” on page 9-5 .
If an L Warning occurs, proceed as follows:
1. If the sample was from a Cavalier King Charles Spaniel, or other breed with a known predis-
position to macrothrombocytosis, perform a manual smear.
2. If the draw was difficult or the platelet value is low, redraw and rerun as this could be platelet
clumping causing the L Warning.
3. Make sure the Lyse tube (yellow) has no kinks, or loose connections at the analyzer or bottle.
4. Make sure the tube is well attached to the connector on the dip tube or bottle cap.
5. Check for bubbles in the Lyse tube. If found, prime the Lyse and rerun the sample.
6. If the Lyse tube is in working order, from the Home screen, select Measure > Run .
7. Select the Change Lyse field, and increase the volume by 0.1 ml.
8. Rerun the sample.
9. If the
L Warning
persists, increase the Lyse by 0.2 ml, then rerun the sample.
10. If the L Warning still persists and there are no Lyse issues, perform a manual smear or send
the sample out.
4.7 Interpreting Results
The VetScan HM5 produces a printed report containing the patient ID, measurement data, numeric
results with flags (if any), and histograms showing the different cell populations.
Complete Blood Count (CBC) parameters are useful in assessing overall wellness of a patient, as well
as identifying and monitoring certain disease states. See “CBC Parameters and Associated Indications”
on page E-1 for information on the various CBC parameters and associated clinical indications.
Note: The new Lyse volume will be used for the current run only, then will
revert to the default setting.
Note: Always check whether the results include warning flags, at the top-
right corner of the HM5 screen or the bottom of the HM5 printouts.