
OPERATION
Axioscope 5/7/Vario
Illumination and contrast methods in reflected light …
ZEISS
03/2018
430035-7344-001
113
•
Turn the knurled button of the aperture diaphragm (
2
) to a medium position (approx. half
open and half closed).
•
Adjust the knurled knob on the field diaphragm (
4
) so that the field diaphragm becomes
visible in the field of view (
A
).
•
Readjust the focus on the edge of the field diaphragm with the focusing drive (
B
) and center
the field diaphragm on the edge of the field of view (
C
) with the centering screws
3
).
•
Now open the field diaphragm enough to make it disappear just past the edge of the field of view
(
D
).
•
To adjust the aperture diaphragm (image contrast), remove an eyepiece from the tube barrel and look
into the barrel or mount an auxiliary microscope instead of the eyepiece.
•
Center the aperture diaphragm with the centering screws (
1
) and adjust the knurled knob
2
) to about 2/3 to 4/5 of the diameter of the objective exit pupil (
E
) for samples
with a medium contrast quality.
In most cases this position of the aperture diaphragm gives the best contrast at almost full resolution,
which is the best compromise for the human eye.
•
Replace the eyepiece. Re-adjust the focus using the coarse and fine focusing drives and set the image
brightness according to the reflected light sample.
NOTE
Never use the aperture diaphragm to regulate the image brightness! Use the control knob on
the external power supply unit to adjust the light intensity!