WPI NANOLITER2020, Инструкция по эксплуатации

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NANOLITER 2020/200704
World Precision Instruments 35
APPENDIX C: FREQUENTLY ASKED QUESTIONS
What is the advantage of using oil back-filled glass micropipettes?
“ Back filling” refers to the process of filling the pipette from the large, non-pulled end.
“ Front filling” describes filling a micropipette through the small, pulled front end of the
pipette. The glass micropipettes are first backfilled completely with mineral oil and secured
to the NANOLITER Injector Head. Then, some mineral oil is dispensed through the tip.
This creates the space and generates the pressure to front fill samples through the tip.
Front filling sample prevents the spillage or loss of costly or scarce sample involved with
back filling samples. When the sample volume is low, first back filling glass micropipettes
with oil and then front filling the sample may be the only option.
Can I use WPI’s old standard controller to run the 300704 Injector head?
WPI NANOLITER2020 Injector Head ( 300704 ) needs the MICRO2T controller
(recommended). WPI’s MICRO4 controller may be used to control the pump. The 300704
Injector Head cannot be used with WPI’s old standard controller.
Can I use same MICRO2T controller that I use with UMP3 pump? Do I need
an additional adapter to use the 300704 pump with my existing MICRO2T
controller?
Yes, the same MICRO2T controller can be used to control the UMP3 pump and the
NANOLITER2020 . No additional adapter is needed. The 300704 connects directly to
MICRO2T controller.
Can I run the pump using a foot switch?
Yes, you can use the 13142 Foot switch. This is not included in the NANOLITER2020
system and is sold separately.
What do I do when glass micropipettes come off after attaching or I am unable
to front fill a sample?
Odorless and colorless mineral oil is used for back filling glass micropipettes. While the
glass micropipette is installed and with gradual spillage over multiple uses, the gaskets
become too slippery to hold glass micropipette in place. Use Kimwipes to soak up oil
spillage inside the injector head, outside the glass micropipettes and to clean the gaskets.
If needed, install a new set of gaskets to resolve the issue.
I followed the instructions manual and I still have questions using the
NANOLITER2020 system. What should I do?
Please contact WPI Technical Support team at (941) 371-1003 or email technicalsupport@
wpiinc.com.