WPI NANOLITER2020, Инструкция по эксплуатации

Страница: 27 / 44

NANOLITER 2020/200704
World Precision Instruments 23
NOTE : The forward arrow shows the injector running in infusion mode to eject oil and
create space inside the micropipette.
3. Press the RUN button to eject a volume of oil.
4. The micropipette tip can be touched very gently and carefully with a Kimwipe to
absorb any remaining oil from the tip.
CAUTION : Tip of the micropipette is extremely delicate and can get damaged
easily.
5. Next place the micropipette tip into the fluid to be loaded. Make sure the tip is
immersed in the liquid, or gas bubbles will collect in the sample.
NOTE : The presence of bubbles inside the micropipettes can result in an inaccurate
volume dispensed.
6. Press the DIRECTION button to set the pump to withdraw. The image of the micropi-
pette on the controller screen should have a green arrow pointing away from the tip.
Fig. 42—The arrow indicates the pump is withdrawing. (Example Parameters: Delivery Rate =
200 nL/sec, Volume Target = 3000 nL, Motor Drive= MAX LOAD)
7. Press the
RUN button. This will draw fluid (front fill) into the micropipette.
NOTE : The backward arrow shows the injector is running in withdrawal mode to fill up
micropipettes through the tip (front fill).
8. The micropipette is ready to inject into target cell or tissue.
9. Press the
DIRECTION button to set the pump to infuse. Now the micropipette can be
placed at the desired injection location and after setting the desired Volume Target
and Delivery Rate, make an injection by pressing the RUN button.
NOTE : The first injection after front filling may be less accurate due to the internal
pump’s mechanical configuration change from reverse to forward motion. We
recommend performing a blank injection right after front filling. (For example, inject
>15 nL and discard the volume.) All subsequent injections will be accurate while the
injector continues to run in the same direction (infuse/inject).
NOTE : The MICRO2T Motor Drive Mode of Max Load is recommended for use with the
NANOLITER2020 Injector.