Warning:
To prevent burn injuries, set the column temperature to Off, and then allow
the column compartment and its components to cool for 60 minutes before touching
them. Monitor the column compartment's internal temperature to ensure that all
components are cool.
Warning:
Observe Good Laboratory Practice (GLP) at all times, particularly when
working with hazardous materials. Consult the Safety Data Sheets regarding the
solvents you use. Additionally, consult the safety representative for your organization
regarding its protocols for handling such materials.
Warning:
To avoid personal contamination with biologically hazardous or toxic compounds,
wear clean, chemical-resistant, powder-free gloves when performing this procedure.
Warning:
To avoid eye injury, use eye protection when performing this procedure.
2.3.1 Installation recommendations for fittings
When making the column compartment plumbing connections, consult the topics in this section
for detailed instructions about assembling and tightening the various fittings used to connect the
column compartment to the system.
These three types of tubing and fittings connect the column compartment to the system,
depending on its configuration:
• SST (stainless steel) tubing and fittings
• MP35N (a nickel-cobalt-chromium-molybdenum alloy) tubing for ACQUITY UPLC H-Class Bio
Series and ACQUITY Arc Bio applications
• PEEK (polymer-based) tubing and fittings for low-pressure outlet connections to detectors or
mass spectrometers
When connecting tubing, heed the following recommendations for installing and tightening fittings.
Recommendations:
• To prevent band spreading, ensure that the tubing bottoms in its connection port before you
tighten the compression fitting.
• Whenever you loosen fittings during maintenance, examine them for cracks, stripped threads,
and deformations.
• Do not reuse fittings with stainless steel ferrules more than six times.
December 4, 2019, 715005052 Version 02 (previously released as Rev.B)
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