Waters 2998, Обзор и руководство по обслуживанию

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December 5, 2017, 715004753 Rev. B
Page 22
Figure 1–7: Filter-time constant comparison
Tip:
Although the peak shape shows some distortion and the signal output is delayed with different
filter-time constants, the peak area remains the same.
1.5 Computing absorbance data points
The absorbance data points are calculated by the detector and then transmitted to the database
(MassLynx or Empower software).
The absorbance calculation is
where
S = sample energy
D = dark energy
R = reference energy
t = elapsed time after start of injection

= wavelength
This value is then filtered as specified.
0 s
1 s
2 s
Time (minutes)
Absor
bance
Absorbance
t
 
Log
10
R

D

–
S
t
 
D

–
-----------------
=