Waters 2998, Обзор и руководство по обслуживанию

Страница: 47 / 94

December 5, 2017, 715004753 Rev. B
Page 47
Tip:
Conditions other than a dirty flow cell can cause decreased lamp intensity. For more
information, refer to Chapter 4 .
Maintaining the flow cell consists of these tasks:
• Flushing
• Removing
• Disassembling and cleaning
• Installing a replacement
3.4.1 Flushing the flow cell
Required tools and materials
• HPLC-grade water
• HPLC-grade methanol
If the flow cell requires cleaning, first flush it with solvent.
To flush the flow cell:
1. Select a solvent compatible with the samples and mobile phases that you have been using.
If you have been using buffers, flush the cell with 10 mL of HPLC-grade water and then
flush with 10 mL of a low-surface-tension solvent, such as methanol.
Requirement:
Ensure that the solvent is miscible with the previous mobile phase.
2. Test the energy by performing the Read energy diagnostic test (see page 53 ).
Note:
If the diagnostic test fails and the has not been used more than 2000 hours or 1 year
from date of purchase (whichever event occurs first), call Waters Technical Service (see
page 45 ).
3.4.2 Replacing the flow cell
Required tools and materials
• 1/4-inch flat-blade screwdriver
• Flow cell
To replace the flow cell:
1. Power-off the detector.
2. Stop the solvent flow.
3. Open the door.
4. Disconnect the column-outlet tubing from the flow cell inlet.
5. Disconnect the waste line from the flow cell outlet.