December 5, 2017, 715004753 Rev. B
Page 93
Unstablized
tetrahydrofuran
(THF), fresh
2.44
2.57
2.31
1.80
1.54
0.94
0.42
0.21
0.09
0.05
Unstablized
tetrahydrofuran
(THF), old
>2.5
>2.5
>2.5
>2.5
>2.5
>2.5
>2.5
>2.5
2.5
1.45
Acids and bases
Acetic acid, 1%
2.61
2.63
2.61
2.43
2.17
0.87
0.14
0.01
<0.01
—
Hydrochloric acid,
0.1%
0.11
0.02
<0.01
—
—
—
—
—
—
—
Phosphoric acid,
0.1%
<0.01
—
—
—
—
—
—
—
—
—
Trifluoroacetic acid 1.20
0.78
0.54
0.34
0.22
0.06
<0.02
<0.01
—
—
Diammonium
phosphate,
50 mM
1.85
0.67
0.15
0.02
<0.01
—
—
—
—
—
Triethylamine, 1%
2.33
2.42
2.50
2.45
2.37
1.96
0.50
0.12
0.04
<0.01
Buffers and Salts
Ammonium
acetate, 10 mM
1.88
0.94
0.53
0.29
0.15
0.02
<0.01
—
—
—
Ammonium
bicarbonate,
10 mM
0.41
0.10
0.01
<0.01
—
—
—
—
—
—
EDTA,
disodium,
1 mM
0.11
0.07
0.06
0.04
0.03
0.03
0.02
0.02
0.02
0.02
HEPES,
10 mM,
pH 7.6
2.45
2.50
2.37
2.08
1.50
0.29
0.03
<0.01
—
—
MES,
10 mM,
pH 6.0
2.42
2.38
1.89
0.90
0.45
0.06
<0.01
—
—
—
Potassium
phosphate,
monobasic
(KH
2
PO
4
), 10 mM
0.03
<0.01
—
—
—
—
—
—
—
—
Potassium
phosphate,
dibasic,
(K
2
HPO
4
),
10 mM
0.53
0.16
0.05
0.01
<0.01
—
—
—
—
—
Table C–5:
Mobile phase absorbance measured against air or water (continued)
Absorbance at specified wavelength (nm)
200
205
210
215
220
230
240
250
260
280
Содержание 2998
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