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Figure 15.
Determining the maximum rate with window value 2
You can define the following settings:
Reaction
– Define whether the reaction is supposed to produce increasing or decreasing signal
levels. There are three options for this setting:
Undefined
(default)
– The reaction can be increasing, decreasing or both (for example, first
increase and then decrease, that is, peaking). The software does not produce any warnings in
either case. It searches for the absolute maximum rate. If the highest absolute rate value is
increasing, it is shown as a positive number. If the maximum rate is decreasing, it will be
presented with the prefix “-”.
Increasing
– The software searches for the maximum increasing rate. The rate is shown as a
positive number. If no increasing rate is found, the result is “NaN” (not a number).
Decreasing
– The software searches for the maximum decreasing rate. The rate is shown as
a positive number. If no decreasing rate is found, the result
is “NaN” (not a number).
Kinetic rate
– Select to view the results in either seconds (s) or minutes (min). The kinetic
rate is always calculated per second but if you select per minute, the time is automatically
converted to minutes after the calculations.
Ignore from beginning
– Ignores a defined number of points (readings), counted from the
first reading.
Ignore from end
– Ignores a defined number of points (readings), counted from the last
reading.
Window
– Select the number of consecutive readings to use for evaluation. The highest
reaction rate for each well is calculated using a sliding window. A window defines how many
measurement points are included in the measurement calculations. The size of this window is
given in the Window parameter box. For example, if the number of measurements is ten and
the Window parameter is three, the system will calculate the first rate using the measurements
