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First define the base value by entering the number of readings from the start in baseline. The base
value is the average of results of the given baseline count from the beginning or end of results. Then
select whether the change from the base value is evaluated as a relative (%) or an absolute value
and define that percentage or value. This change is then added to the base value to create a required
change value. The average of the sliding window is compared to the limit. The result is the exact
interpolated time where the given change occurs.
You can define the following settings:
Ignore from beginning
– Ignores a defined number of points (readings), counted from the first
reading.
Ignore from end
– Ignores a defined number of points (readings), counted from the last reading.
Window
– The number of consecutive readings to use for evaluation.
Reaction
– Define whether the reaction is supposed to produce increasing or decreasing signal
levels. There are three options for this setting:
Undefined
– Searches for the defined change in signal, regardless of the rate polarity
(positive or negative), and returns the corresponding time.
Increasing
– Searches for the increasing defined change in signal, and returns the
corresponding time. If no increasing rate is found, the result is “NaN” (not a number).
Decreasing
– Searches for the decreasing defined change in signal, and returns the
corresponding time. If no decreasing rate is found, the result is “NaN” (not a number).
Baseline readings
– The Baseline readings parameter is the number of initial readings,
calculated from the beginning (
first
) or end (
last
) of the measurement, used for the baseline
calculation. The baseline can also be zero, which means that the comparison will be made
against zero. In the Baseline readings the software chooses as many measurement points as
exist in the baseline and calculates the mean thereof. The mean equals the baseline value. If
last
is selected, the change is searched starting from the end of the measurement.
Readings from
– There are two options for this setting:
Beginning
(default) or
End
.
Change
– The change in signal is specified and compared to the baseline.
Change type
– The relative change from the baseline in percent or the absolute change. The
default is
Absolute
.
Maximum of well
The maximum of well is used to search for the maximum measurement value in each well.
