78
Performance specifications
Table 16.
Photometry
This section provides the performance specifications at typical laboratory conditions (22°C, relative
humidity 40%) for the relevant measurement technique and other instrument capabilities.
Performance specifications / Photometry
Optical system
Quartz-halogen lamp (Osram 64222, 6V/10W), interference filter (in the filter
wheel), fiber, fiber end optics, photodetector, signal processing
Wavelength range
340
–850 nm
Filters
8-position filter wheel
The instrument is delivered with the following standard filters installed: 405 nm;
450 nm, and 620 nm. Additional filters can be ordered separately.
Half-bandwidth of filters
3
–9 nm
Wavelength accuracy
2 nm
Detector
One silicon photodetector
Linearity
(405 nm)
0
–3 Abs,
2%, 96-well plate
0
–2.5 Abs,
2%, 384-well plate
Measurement range
0
–6 Abs
Absorbance resolution
0.001 Abs
Accuracy
(405 nm, normal mode)
1% (0
–3 Abs) or
0.003 Abs which ever is greater
Precision
(405 nm, normal mode)
CV
0.2% (0.3
–3 Abs)
Startup time
Approx. 30 s
Measurement time
7 s (96-well plate), fast mode
13 s (96-well plate), normal mode
16 s (96-well plate), fast mode, dual wavelength
13 s (384-well plate), fast mode
34 s (384-well plate), normal mode
28 s (384-well plate), fast mode, dual wavelength
Long-term stability
Instrument is stable due to autoblanking procedure.
Table 17.
Incubator
Performance specifications / Incubator
Temperature range
A4
C to 50
C in increments of 1
C
Liquid warm-up time
Liquid warm-up time < 40 min from 25
C to 37
C, 96-well plate, 200 µl
water/well
Uniformity
± 1
C across the plate (at 37
C)