27
Note!
Blank subtraction is made automatically if the plate layout includes blanks. The blank
used in the calculations is always an average of the blanks included in the layout.
You can determine the sample type in
Well type
. The sample types are
Blank
,
Calibrator
,
Control
,
Unknown
or
Empty
.
Note!
The results will not be calculated for the
Empty
sample type.
Note!
The calibrator concentrations are programmed in the
Plate layout
window. You can
define calibrator concentrations when you create a calibrator series or activate a single
calibrator. A calibration concentration of 0 is not accepted.
Fill direction
can be either to the right or downwards from the active well in the
Plate layout
window.
Fill replicate direction
can be either the same or different from the fill samples direction in the
Plate
layout
window.
You can define the number of samples belonging to the group, for example, the number of different
calibrators or the number of different unknown samples in
Number of
. You define the number of
replicates for each sample type in
Replicate
.
Note!
The
Replicate
number is the same for samples, for example, for different controls when
using
Add series
starting from the well programming. Use the
F3
(
Edit
) key to program each
sample individually, if a different replicate number for the samples are needed.
When filling the plate with the
Add series
function, note that adding a group of samples of the same
type to another location will delete the first group. If you want to add the same well type into several
separate locations on the plate layout, use the
Edit
function.
For instructions on how to program the plat
e layout, refer to “Programming a plate layout”.
Preprocessing parameters
You can define the preprocessing parameters in the
Preprocessing
window.
Preprocessing includes two different types of data processing:
precalculations
and
kinetic
calculations
.
