Thermo Scientific IonPac CG16, Руководство по эксплуатации продукта

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Product Manual for IonPac CS16 Page 38 of 65
Under gradient conditions, from 6 mM up to 55 mM methanesulfonic acid, using an Eluent Generator, peaks # 3 and 4,
ammonium and ethanolamine, are well separated, and the total run time is even a few minutes shorter than before. Starting
the elution with a low concentration acidic eluent and using the column at high temperature, 60 °C, aids the separation of
these two components. The acid concentration in the eluent is then increased in a gradient to speed the analysis, having the
added benefit of sharpening the later-eluting peaks and improving their minimum detection levels. The eluent contained no
organic solvent, so the suppressor was used in the eluent recycle mode. Note that the suppressor is placed outside the
chromatography oven when temperatures exceed 40 °C as they do here.
Column:
IonPac CS16 3-mm Analytical Column (no guard)
Eluent: 6 mM MSA, gradient from 0 to 13 min to
8 mM MSA, gradient from 13 to 25 min to
55 mM MSA
Eluent Source: EG40
Flow Rate: 0.5 mL/min
Inj. Volume: 25 µL
Detection: Suppressed conductivity
CSRS 300 2-mm outside oven
AutoSuppression
®
Recycle Mode
0 5 1 0 1 5 2 0 2 5
0 .1
1 .3
2 .5
µ S
1
2
3
4
5
6
7
8
9
1 0
1 1
1 2
Analyte mg/L
1. Lithium 0.05
2. Sodium 0.20
3. Ammonium 0.42
4. Ethanolamine 0.50
5. Methylamine 0.50
6. Potassium 0.50
7. Dimethylamine 1.00
8. 5-Amino-1-pentanol 2.00
9. Morpholine
2.00
10. Trimethylamine 1.50
11. Magnesium 0.25
12. Calcium 0.50
Figure 22 Continued
Gradient Elution of Common Cations and Amines at 60 °C
Document No. 031747-05 © 2010 Dionex Corporation December 2010