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Installing the Reagents
1.
Remove the caps from the 6.8 and 7.3 bottles.
2.
Insert the tubing connectors into the bottles and push the caps onto
the bottles.
3.
Place the bottle assembly in the lefthand side of the reagent
compartment feeding the tubes through the caps into the solutions.
4.
Date the buffer pack label a maximum of 30 days ahead.
5.
Remove the CD pack from the neck of a wash bottle. Remove the
bottle cap.
6.
Insert the tubing connectors into the bottle and push the cap onto
the bottle.
7.
Place the bottle to the right of the buffer bottles feeding the tube
through the cap into the solution.
8.
Check that the waste bottle is in position.
9.
Check that the neck of the waste bottle is positioned underneath
the rubber cap. The waste cap spout should be inside the neck of
the waste bottle.
Installing the Gas Cylinders
CAUTION:
The instructions given are for Siemens gas cylinders and
regulators and we recommend the use of these at all times. If you use
other cylinders and regulators they must meet the following
specfication:
•
Only certified value gas cylinders should be used.
For calibration gas the value should be 5% CO
2
(acceptable range
4 to 6%) and 12% O
2
(acceptable range 10 to 14%), balance N
2
.
For slope gas the value should be 10% CO
2
(acceptable range 8 to
12%) and 0% O
2
(acceptable range 0 to 2%), balance N
2
.
The gas values used must be entered (see Page 5-2,
Entering Gas
Values
).
•
The regulator must have a low diffusion diaphragm to prevent
selective diffusion and alteration of the gas values. All fittings must
be leak free. The regulator must have a low flow precision needle
valve for gas flow adjustment and supply 4
±
1 psi at a flow of up to
20 cc/minute. When the needle valve is closed the output psi must
not rise by more than 2 psi compared to the ‘open’ output.
•
The tubing used must not allow selective diffusion of CO
2
, O
2
or N
2
.
Siemens tubing is designed to maximize through-flow rates,
reducing the diffusion rates. The accuracy of the 248 is adversely
affected by the use of diffusive tubing.
•
Siemens assumes no liability for performance when non-standard
equipment is used.
Page F-4
248 Operator’s Manual
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