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PippinHT Operations Manual
12-13
460005 Rev G
12.4.4 Optical Monitoring
Optical monitoring is provided for both cassette/nests. The cassette/nests screens can
be toggled by clicking the tabs. There are two monitoring views for each nest; and
electropherogram view, and an image view. If a fluorescent marker is detected, a
correctly identified marker will have a blue line through the peak.
Important! Fail-Safe for Missed Peak Detection:
Each cassette definition includes a time
value by within which all peaks should be called. If the run reaches that specified duration, and
the peaks have not been detected, the software decreases the base-to-threshold value by 25%
and re-scans for peaks. It will repeat this with an additional 25% decrease in base-to-threshold if
peaks are still not detected. If peaks are finally detected, the run will proceed as usual.
If peaks
are still not detected, the lane will switch to 'idle'.
This will prevent sample loss. All others
lanes will proceed with the run. Users can then choose to manually elute their sample from that
lane once the run is complete (see next section, 12.5 for the manual procedure).
Correctly called
peaks with be marked
with blue lines and
the marker size
numbers.
