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PippinHT Operations Manual
9-3
460005 Rev G
7.
Remove all buffer
from elution modules and replace with 30μl of fresh
electrophoresis buffer
. Replenishing the buffer ensures proper electrophoretic
continuity. When refilling empty modules, place tip all the way into the module and slowly
fill from the bottom, withdrawing the pipette tip while dispensing, and taking care not to
generate any bubbles that can occlude the current path. Use spare buffer that has been
supplied with the cassette package, or pipette from the buffer chambers.
Important!
Make sure that the pipette tips extend all the way to the bottom of the elution modules
without sealing the elution port opening. If the tips seal the port opening, it will be extremely
difficult, or impossible, to empty and refill the elution module completely. Test tip fit using the
empty rinse cassette supplied with the instrument.
8.
Seal the elution wells with the adhesive tape strips.
Tape for sealing the elution wells
are supplied with cassette packaging. Long elutions will cause the elution wells to
overflow if not sealed.
Place tape over the elution wells and rub firmly to fix the tape in
position. For best results, rub the tape at the boundary of the well with a smooth hard
object such as the back end of a lab marker pen.
9.
Place the cassette(s) onto the PippinHT nest(s)
Remove all buffer
from elution wells
and replace with
30μl of fresh buffer.
