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PippinHT Operations Manual
10-3
460005 Rev G
Failure due to buffer levels:
A low level of buffer in any of the buffer chambers can also result in continuity test failures.
In the elution channel continuity test, an empty elution well will also result in a failed test.
When there is a continuity test failure, user should inspect the buffer chambers for the
affected lane-pairs. A properly filled chamber will have the appearance of a small bubble at
the top of the chamber. A low-level of buffer will have the appearance of an obviously larger
bubble when compared to adjacent chambers. If this is the case, users should add
additional buffer until the level looks approximately similar. If the elution channel has failed,
it is also prudent to replace the buffer in the elution well. Buffer trouble shooting is
summarized in
Figures 10.1 and 10.2
.
Figure 10.1.
Failed Separation continuity test, lane-pair 3/4. Check upper and lower buffer chamber buffer levels.
Figure 10.2.
Failed Elution continuity test, lane-pair 3/4. Check upper and middle buffer chamber buffer levels,
and replace elution well buffer.
