R-Biopharm Rida Cycler, Руководство пользователя

Страница: 86 / 87

RIDA
®
CYCLER
Version 1.0
|
User manual
86
Glossary
Analytical Accuracy the difference between the experimentally measured and actual
concentrations.
Analytical Repeatability: precision of the assay within the same samples repeatedly
measured in the same assay. Also, referred to intra-assay
variance it is expressed as the SD for the Cq variance or CV of
the copy number/ concentration variance.
Analytical Reproducibility the variation in results between runs or different laboratories. It
is also referred to as inter-assay variance and is expressed as
the SD or CV of copy number or concentration. As Cq’s
typically vary between runs the reporting of inter run variation
is not appropriate.
Analytical sensitivity the minimum number of copies in a sample that can be
measured accurately with an assay.
Analytical specificity assay detecting the specific target sequence rather than
another, nonspecific, target. Use of NTC’s helps determine
analytical specificity.
Hydrolysis probes are short oligonucleotides with a fluorescence reporter dye at
the 5’ end and a quencher molecule at the 3’ end. When the
probe is intact, the close proximity of the reporter dye to the
quencher results in little fluorescence being detected. During
extension, the polymerase will cleave the probe through
exonuclease activity separating the reporter dye from the
quencher.
Limit of Detection (LoD) the LoD is the minimum concentration that can be detected
with reasonable certainty (typically 95 % probability).
Linear dynamic range is the highest to the lowest quantifiable copy number
determined by means of a standard curve
Magnetic induction when a conductor such as a metal is exposed to a magnetic
field it produces a circular electric current, also known as an
eddy current, in the conductor. Due to the resistance of the
conductor it becomes hot. In the RIDA
®
CYCLER instrument the
rotor is the conductor that becomes hot under as it is exposed
to a magnetic field.
,
.