R-Biopharm Rida Cycler, Руководство пользователя

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RIDA
®
CYCLER
Version 1.0
|
User manual
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9 Starting the Run ...............................................................................................................40
9.1 Samples Editor ............................................................................................................................... 42
9.1.1 Well layout .................................................................................................................................... 42
9.1.2 Filling cells ................................................................................................................................... 42
9.1.3 Colors ........................................................................................................................................... 43
9.1.4 Name ............................................................................................................................................. 43
9.1.5 Type .............................................................................................................................................. 44
9.1.6 Sample Concentrations .............................................................................................................. 46
9.1.7 Multiplex Standards .................................................................................................................... 48
9.1.8 Linking an assay to a sample ..................................................................................................... 48
9.1.9 Optional columns ........................................................................................................................ 49
9.1.10 Import samples .......................................................................................................................... 49
9.1.11 Sample editor warnings ............................................................................................................ 50
9.1.12 Lock down samples editor ....................................................................................................... 50
9.1.13 Information ................................................................................................................................. 50
10 Creating templates ........................................................................................................52
10.1 Create a template.......................................................................................................................... 52
10.2 Opening a template ...................................................................................................................... 52
11 During a run ...................................................................................................................52
11.1 Modifying a profile during a run (optional) ................................................................................ 53
11.2 Data ................................................................................................................................................ 53
11.3 Messages ...................................................................................................................................... 54
12 Analysis ..........................................................................................................................54
12.1 Cycling analysis ........................................................................................................................... 55
12.1.1 Graph types ................................................................................................................................ 55
12.1.2 Cycling analysis parameters .................................................................................................... 57
12.2 Cycling analysis results table ..................................................................................................... 59
12.2.2 Floating windows ...................................................................................................................... 60
12.3 Standard curve using absolute quantification analysis ........................................................... 61
12.3.1 Efficiency calculation ................................................................................................................ 62
12.3.2 Standard curve results table .................................................................................................... 63
12.3.3 Cycling analysis for standard curves ..................................................................................... 64
12.4 Absolute quantification ............................................................................................................... 65
12.4.1Sample results table .................................................................................................................. 65
13 Reports ...........................................................................................................................66
13.1 Report configuration .................................................................................................................... 66
13.2 Report preview .............................................................................................................................. 67
13.2.1 Run properties ........................................................................................................................... 67
13.2.2 Samples ...................................................................................................................................... 68
13.2.3 Analysis ...................................................................................................................................... 68
13.3 Report options .............................................................................................................................. 69