R-Biopharm Rida Cycler, Руководство пользователя

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RIDA
®
CYCLER
Version 1.0
|
User manual
60
Well: the order of the well numbers will depend on the grouping of the samples as the name of
the sample order can be alphanumeric (grouped) or numeric (ungrouped).
Cq: the quantification cycle value for each sample, which is dependent on the cycle threshold
set.
Efficiency: the amplification efficiency is calculated for each sample using the LinRegPCR
algorithm described by Ramakers et al. (2003). Using the slope of the linear regression line
from the calculated W-o-L, efficiency is calculated as; E=
〖
10
〗
^slope-1.
Alternatively, a single efficiency value can be determined for a selected assay using the
Standard Curve Analysis method.
R2: the r-squared value is a quality measure of the linear regression used to calculate
amplification efficiency (values > 0.98 are acceptable).
Result: any issue related to the quality of the sample is reported in the results column. For
example, if a Cq value cannot be determined.
12.2.1 Cycling analysis sample table selector
The result table can be compressed by hiding the individual sample results using the triangle
at the top of each replicate row or selecting the Full Collapse option in the
Sample table
selector for the whole table. Right click the Sample table selector to view the different table
sorting options.
To sort the samples in replicates using an alphanumeric order use the Group by this column
option. Otherwise select the Ungroup option to have the samples displayed in order of well
number.
The individual samples can be sorted in Ascending or Descending order for any of the columns
for both grouped and ungrouped tables. Use this option to determine the range of Cq or
efficiency values by displaying the highest to the lowest values.
Fig. 53: Cycling analysis sample table
12.2.2 Floating windows
Under some circumstances it may be necessary to view either the cycling analysis graph or
cycling analysis parameters in parallel with another analysis type. For example, you may want
to observe what a change in cycle threshold has to reaction efficiency using a standard curve.
The floating window option allows you to achieve this multiple analysis window view.
Select the Pop out copy of panel into floating window icon.